Synthesis and characterization of antimicrobial peptides and isolation of phytochemical constituents of medicinal plant Asif Ahmad 1 , Farzana Shaheen 1 1 HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan Natural products are diverse molecules reported to have tremendous medicinal benefits. They are life saver for all the living beings present in various forms and can be obtained from various sources. Peptides are among those naturally occurring medicinally important natural products which are not only bioactive but also have selective mode of action. They can be obtained from various terrestrial as well as aquatic sources. Specifically peptides obtained from skin secretion of frogs are reported to have great antimicrobial potential. They are reported to be highly effective against a broad range of antibiotic resistant microorganisms by disrupting their plasma membranes. The therapeutic potential of these AMPs is not limited, they are also reported to possess some anticancer activities as well. But the only problem with that peptide therapeutics is that they can easily be affected by proteolytic enzymes. This limitation can be overcome by introducing some structural modification in the basic skeleton of these peptide molecules or by introducing some unusual amino acid residues in the chain. The current study is based on exploration of naturally occurring medicinal agents from Medicinal plant and synthesizing a new series of antimicrobial peptide derivatives through Fmoc assisted solid-phase peptide synthetic methodology. The structures of all the compounds were confirmed by using different spectroscopic techniques and were checked against different fungi and bacterial strains in order to check their antimicrobial potential. Details of this study will be discussed in the poster presentation. References 1. Urbán E, Nagy E, Pál T, Sonnevend A, Conlon JM. Activities of four frog skin-derived antimicrobial peptides (temporin-1DRa, temporin-1Va and the melittin-related peptides AR-23 and RV-23) against anaerobic bacteria. Int J Antimicrob Agents. 2007 Mar;29(3):317-21. doi: 10.1016/j.ijantimicag.2006.09.007. Epub 2006 Dec 28. PMID: 17196372. 2. Pushpanathan M, Gunasekaran P, Rajendhran J. Antimicrobial peptides: versatile biological properties. Int J Pept. 2013;2013:675391. doi: 10.1155/2013/675391. Epub 2013 Jun 26. PMID: 23935642; PMCID: PMC3710626.
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