Evaluation of anti-inflammatory activity of ethanol extracts of leaves and bark of Dialium ovoideum Thwaites Chathurangani D.A.U. 1 , Dinusha N. Udukala 1 , and Medha J. Gunaratna 2 1 Institute of Chemistry Ceylon, College of Chemical Sciences, Sri Lanka, 2 Department of Chemistry, Faculty of Science, University of Kelaniya, Sri Lanka. Inflammation is a response to injury characterized by cell and exudate accumulation in irritated tissues that provide protection from further damage where the symptoms of inflammation include redness, joint pain, swollen joints, stiffness, and reduced joint function. The development of potent anti-inflammatory drugs from natural products with fewer side effects has gained more attention than synthetic drugs. Dialium ovoideum Thwaites, an endemic plant in Sri Lanka, is used in traditional medicine to treat skin infections, clean wounds, and act as an antidote for snake bites. The purpose of this study is to examine the anti-inflammatory effects of ethanol extracts of the leaves and bark of D. ovoideum Thwaites. The leaves underwent sequential extraction with hexane, ethyl acetate, and ethanol, while the soxhlet extraction method was utilized to prepare the ethanol extract of the bark. The human red blood cell (HRBC) membrane stabilization assay was utilized as a simple and rapid method to evaluate anti-inflammatory activities. Aspirin, a commercially available drug was used as the reference standard to compare the anti-inflammatory activities of the ethanol extracts of the leaves and bark. The ethanol extract of the bark of D. ovoideum Thwaites exhibited higher anti-inflammatory activity than the ethanol extract of the leaves. The IC 50 values for the heat-induced HRBC membrane stabilization assay of ethanol extracts of bark and leaveswere 425.7±9.3 µg/mL and 525.0±7.6 µg/mL, respectively, which is comparable to aspirin (162.0±7.9 µg/ mL). The present study demonstrates significant (p<0.05) anti-inflammatory properties of ethanol extracts of the leaves and bark of D. ovoideum Thwaites. References 1. Bulugahapitiya, V. P.; Rathnaweera, T. N.; Manawadu, H. C. Phytochemical Composition and Anti-Oxidant Properties of Dialium ovoideum thwaites (Gal Siyambala) Leaves. J. Minor Fruits, Med. Aromat. Plants. 2020 , 6 (1), 13–19. 2. Bulugahapitiya, V. P.; Rathnaweera, T. N.; Wijayaratne, W. M. D. G. B.; Manawadu, H. C. Cytotoxicity and Antimicrobial Activity of Dialium ovoideum thwaites , an Endemic Plant in Sri Lanka. Asian J. of Med. Biol. Res. 2020 , 6 (2), 316–320. 3. Uduwela, U. D. H. K.; Deraniyagala, S. A.; Thiripuranathar, G. Antioxidant and Anti-Inflammatory Potential of the Aqueous Extract of the Peel of a Sri Lankan Variety. World J. Pharm. Res. 2019 , 8 (2), 154–166.
P16
© The Author(s), 2023
Made with FlippingBook Learn more on our blog