Investigation of in vitro anti-inflammatory activity of extracts and compounds from Garcinia quaesita fruit seed
Medha Jaimini Gunaratna , M. W. Sajani Hansagee Department of Chemistry, University of Kelaniya, Sri Lanka.
Garcinia quaesita Pierre is an endemic plant in Sri Lanka. This plant's culinary and medicinal qualities make it remarkably potential for future exploitation. The main objective of this research was to investigate in vitro anti-inflammatory activity of Garcinia quaesita fruit seed. The phytoconstituents in the dried seeds of the Garcinia quaesita were extracted using sequential Soxhlet extraction using hexane, ethyl acetate, and ethanol, respectively. Crystals formed in the oils of hexane and ethyl acetate extracts after a certain period of standing following the extraction. Each extract was evaporated to dryness to obtain seed oils. The crystals and the seed oils were purged with nitrogen and stored at -20 ⁰ C until further use. To study in vitro anti-inflammatory effects of the crystals and the seed oils of fruit seeds of Garcinia quaesita , Human Red Blood Cell (HRBC) membrane stabilization activity was assessed. The in vitro HRBC membrane stabilization activities of hexane extract (391.55±2.70 μg/ml), ethyl acetate extract (302.29±3.50 μg/ml), ethanol extract (236.2±1.09 μg/ml), hexane crystal (232.8±4.34 μg/ml) and ethyl acetate crystal (584.5±1.53 μg/ml) of Garcinia quaesita fruit seed were higher compared to the O -acetyl salicylic acid standard (674.32±25.40 μg/ml). To further investigate the active compounds responsible for anti-inflammatory activity, crystals and extracts of hexane and ethyl acetate were fractionated using column chromatography to obtain six major compounds C1-C6. Each purified compound was subjected to subsequent anti-inflammatory testing to identify the compounds exhibiting the highest activity. The in vitro HRBC membrane stabilization activities of compound C1 (222.51±3.59 μg/ml), compound C4 (243.17±12.90 μg/ml), compound C3 (314.79±11.95 μg/ml), compound C6 (519.71±24.01) of G. quaesita fruit seed were higher compared to the O -acetyl salicylic acid standard (674.32±25.40 μg/ml). References 1. Pushpakumara, D.K.N.G., Gunasena, H.P.M. and Singh, V.P., . Underutilized Fruit Trees in Sri Lanka. World Agroforestry Centre. South Asia Regional Office, New Delhi, Pushpakumara, D.K.N.G., Medagoda, I. and Eeswara J.P. Chapter 10 Goraka Garcinia quaesita Pierre, 2007 ,pp.303-322. 2. Daïou, H.; Gael Njini, N.; Ribeiro Souza, H.; Yoshikawa, A.; Yadang Sabine Adéline, F.; Kombou Eleonore Magloire, T.; Paula Girol, A.; Télesphore Benoît, N.; Agbor Agbor, G.; MAKEMBE Adèle, E. Antioxidant Capacity and Anti-Inflammatory Activity of Garcinia Kola (Heckel) Stem Bark Extracts. ~ 16 ~ International Journal of Herbal Medicine 2021 , 9 (6), 16–27.
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