Single-platform, attomolar detection of Alzheimer’s disease biomarker by flexible SERS sensor Ankita Jaiswal 1 ,Tania K. Naqvi 2 , Prabhat K. Dwivedi 2 , Sandeep Verma* 1,2 1 Department of Chemistry, Indian Institute of Technology Kanpur, India, 2 Center for Nanosciences, Indian Institute of Technology Kanpur, India Alzheimer’s disease detection at pre-symptomatic stage is crucial for superior healthcare management. Herein, we developed a Surface Enhanced Raman Spectroscopy (SERS) based sensor for detection of Alzheimer’s disease biomarker (β-Amyloid peptide). 1 Polyacrylonitrile-based nanofiber mats, containing purine ligand, were prepared by electrospinning followed by functionalization with silver nanoparticles (AgNPs). Optimized biosensor (P 3 /AgNPs) was chosen for the detection of β-Amyloid peptide (Aβ 1-42 ), a biomarker of Alzheimer’s disease; human insulin (HI); bovine serum albumin (BSA) and a simulated biomarker of artificial cerebrospinal fluid (CSF) to assess selectivity. Limit of detection (LoD) was found to be 76 × 10 -18 M, 26 × 10 -18 M and 2.0 × 10 -17 M for Aβ 1-42 , HI and BSA, respectively. Thus, we demonstrate the development of an ultra-sensitive and flexible SERS biosensor for detection of multiple biomarkers on a single platform with excellent sensitivity, selectivity and stability. 2
Fabrication and detection scheme of purine-based SERS sensor. References 1. Zhang, X.; Liu, S.; Song, X.; Wang, H.; Wang, J.; Wang, Y.; Huang, J.; Yu, J. ACS Sensors 2019 , 4 , 2140–2149. 2. Jaiswal, A.; Naqvi, T. K.; Dwivedi, P.K.; Verma, S. Chem. – An Asian J. 2023 , 18 , 1-7.
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