Antimicrobial peptide dendrimers for targeting cancer and microbial infections Arif Iftikhar Khan 1 , Shahzad Nazir 1 , Aaqib Ullah 1 , Muhammad Nadeem ul Haque 1 , Rukesh Maharjan 1,2 , Shabana U. Simjee 1,2 , Hamza Olleik 3 , Elise Courvoisier-Dezord 3 , Marc Maresca 3 , Farzana Shaheen 1 1 Third World Center for Science and Technology, University of Karachi, Pakistan, 2 Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan, 3 Aix-Marseille Univ, Marseille, France Temporin SHa, isolated from the skin of Sahara frog Pelophylax saharicus ,was identified as a potent inhibitor of Gram-positive and Gram-negative bacteria,yeasts, fungi, and protozoa, as well as anti-parasitic activity against promastigote and the intracellular stage (amastigote) of Leishmania infantum. Our group had previously synthesized potent anti-cancer linear analogs of temporin SHa. These analogs manifested antiproliferative action against cancerous as well normal cell lines. We developed the several branched analogs of previously synthesized potent analogs of temporin SHa. Herein, we report the synthesis, characterization and anticancer along with antimicrobial activity of dendrimers of temporin SHa, which include dimer [G10a] 2 -SHa , trimer [G10a] 3 - SHa and conjugate of dimer with the lipophilic polymer molecule JEFF-[G10a] 2 -SHa for the very first time. While the structural features of peptide dendrimers such as size, morphology, zeta potential, as well as antimicrobial and anticancer efficacy were also evaluated. References 1. Shaheen, F., M. Nadeem-ul-Haque, et al. (2018). "Synthesis of breast cancer targeting conjugate of temporin-SHa analog and its effect on pro-and anti-apoptotic protein expression in MCF-7 cells." Peptides 106 : 68-82. 2. Khan, A. I., Nazir, S., Ullah, A., Haque, M. N. u., Maharjan, R., Simjee, S. U., Olleik, h., Dezord, E. C., Maresca, M., and Shaheen, F. (2022). Design, Synthesis and Characterization of [G10a]-Temporin SHa Dendrimers as Dual Inhibitors of Cancer and Pathogenic Microbes. Biomolecules, 12(6), 770.
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