Design and synthesis of analogues of anticancer and antiseizure peptide Sana Kiran 1 , Humaira Hassan 2 , Sadiq Noor Khan 1 , Munazza Raza Mirza 2 , Farzana Shaheen 1 1 Third World Center for Science and Technology, University of Karachi, Pakistan, 2 Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan Peptides are actively involved in different biological functions, importantly contribute to human health, and the use of peptides as therapeutics has a long successful history in disease management. In the present study, new analogues of the antiseizure natural product peptide CP1 were synthesized by N -terminal modification, e.g. branching, and fatty acid conjugation with the natural product to improve the efficacy and potency of the peptide. The natural product peptide sequence IIGTILGLLKSL-NH 2 isolated from the Chartergellus communis social wasp venom displayed both antiseizureand anticancer activities. All the peptides were synthesized by using the solid phase Fmoc strategy. Purification of the peptides was performed by using reverse-phase HPLC and by precipitation of peptides by methanol. Different characterization techniques were used to elucidate the structure of peptides. The analogues of the CP1 peptide were found to be active against the MCF-7 breast cancer cell line. Design, synthesis, and structural studies of peptides will be presented in this poster. References 1. Soares S, Lopes KS, Mortari M et al (2022) Antitumoral potential of Chartergellus-CP1 peptide from Chartergellus communis wasp venom in two different breast cancer cell lines (HR+ and triple-negative).Toxicon216: 148-156. 2. Lopes KS, Quintanilha MVT, de Souza ACB et al (2021) Antiseizure potential of peptides from the venom of social wasp Chartergellus communis against chemically-induced seizures. Toxicon 194: 23-36. 3. Deo SVS, Sharma J, Kumar S (2022) GLOBOCAN 2020 report on global cancer burden: challenges and opportunities for surgical oncologists. Ann. Surg. Oncol 29: 6497–6500.
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