A localized surface plasmon resonance aptasensor for rapid and selective detection of the SARS-CoV-2 antigens Tyra Lewis, Erin Giroux, Dr. Sanela Martic Department of Forensic Science, Environmental and Life Sciences Program,Trent University, Canada With the emergence and rapid spread of the SARS-CoV-2 virus, the development of reliable methods for detection are important for maintaining general health and well-being globally. Conventional methods for viral detection, such as nucleic acid-based polymerase chain reaction (PCR) can be costly, time-consuming, and accompanied by high false -negative and -positive rates. 1 Alternative methods are therefore needed to improve the overall reliability and response time of results. The SARS-CoV-2 spike (S) glycoproteins play a vital role in the function of the virus and can be used as diagnostic biomarkers of viral infections. 1 For sensing applications, aptamers are suitable high-affinity binding partners for specified targets, including viral antigens. 1 Localized surface plasmon resonance (LSPR) is an analytical technique that can generate real-time, rapid results within minutes, which is also beneficial for point-of-care testing. Herein, a LSPR aptasensor was fabricated on a gold nanoparticle-streptavidin-biotin surface and using aptamers for the detection of SARS-CoV-2 antigens. 2 The S1 aptamer selectively bound the S1 antigen, with high affinity. Spiked samples achieved >90% recovery with the S1 aptasensor, and the sensor exhibited excellent shelf-life stability. Data indicate that LSPR is a viable analytical tool for measuring SARS-CoV-2 related aptamer-antigen interactions and can be applied to other viral or non-viral antigen targets. References 1. Song, Y., Song, J., Wei, X., Huang, M., Sun, M., Zhu, L., Lin, B., Shen, H., Zhu, Z., Yang, C. Anal. Chem. 92 (2020) 9895–9900. 2. Lewis, T., Giroux, E., Jovic, M., Martic, S., Analyst 146 ( 2021) 7207-7217.
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