Biocompatible hydrogels for oral fluid sampling and biomarker detection in periodontal disease diagnosis -a proof-of-concept Adelaide Mensah 1 , John Callan 1 , Murtaza Tambuwala 2 , Aaron J. Courtenay 1 * 1 Ulster University, School of Pharmacy and Pharmaceutical Sciences, Northern Ireland, UK, 2 Lincoln Medical School, Universities of Nottingham and Lincoln, UK Biofluid sampling techniques in periodontal disease diagnostics pose a challenge to optimum diagnostic decisions. A range of hydrogels have been developed from biocompatible polymers as oral fluid sampling devices for periodontal disease diagnostics. As a proof-of-concept, the hydrogels' uptake of a macromolecule (FITC- dextran 150 kDa) was analysed. The rheological characteristics and swelling capacity of the hydrogels were also evaluated. All hydrogels efficiently took up FITC-dextran molecules. Aqueous blends of formulations had a shear modulus ranging from 300 to 1700 Pa. The swelling capacity revealed that these formulations had a percentage increase in weight from 100% to 3000%. Hydrogels exhibited desirable macromolecule uptake potential and were characterised further based on rheological assessment and swelling profile. References 1. Dental, S., 2014. Prevention and Treatment of Periodontal Diseases in Primary Care Dental Clinical Guidance. Scottish Dental Clinical Effectiveness Programme. 2. Patel, J., Wallace, J., Doshi, M., Gadanya, M., Yahya, I.B., Roseman, J. and Srisilapanan, P., 2021. Oral health for healthy ageing. The Lancet Healthy Longevity , 2 (8),e521-e527.Gul, S. S., Abdulkareem, A. A., Sha, A. M. & Rawlinson, A. 2020. Diagnostic accuracy of oral fluids biomarker profile to determine the current and future status of periodontal and peri-implant diseases. Diagnostics, 10 , 838.
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