Application of the UHPLC-q-TOF-MS and molecular networking in revealing chemo-taxonomical markers of Viscum combreticola Engl. and Viscum album L Babra Moyo 1,3 , Nikita T Tavengwa 2 , Ntakadzeni E Madala 1 1 Department of Biochemistry and Microbiology, Faculty of Science, Engineering and Agriculture, University of Venda, South Africa, 2 Department of Chemistry, Faculty of Science, Engineering and Agriculture, University of Venda, South Africa, 3 Department of Food Science and Technology, Faculty of Science, Engineering and Agriculture, University of Venda, South Africa A metabolic profiling approach employing the UHPLC-q-TOF-MS and classical molecular networking was used to investigate the polyphenol profile differences between Viscum combreticola Engl. (African mistletoe) and Viscum album L. (European mistletoe). The findings of this study revealed that V. combreticola was predominantly characterized by a diverse hydroxycinnamic acid derivatives profile, mainly comprising of structural isomers of monoacylated, homodiacylated, heterodiacylated and heterotriacylated chlorogenic acids. Remarkably, a succinoyl and two new hydroxybenzoyl containing chlorogenic acids were identified for the first time in Viscum species in this study. In contrast, V. album contained flavonoids (flavonols, flavanones and flavones) showing various glycosylation patterns, where the majority of these compounds were acylated to a 3-hydroxy- 3-methylglutaroyl moiety. Moreover, V. combreticola also displayed a different flavonoid profile that exhibited different glycosylation patterns from that observed in V. album . As such, the distinct polyphenol profiles of these two Viscum species can serve as a chemo-taxonomical marker of these plants. Additionally, this study demonstrated that molecular networking is a powerful tool in visualizing differences between the chemical composition of different samples. The diverse polyphenol profile of these two Viscum species might be attributed to their unique metabolism which could be influenced by their genetic make-up, thereby, allowing them to survive various biotic and abiotic stresses. References 1. Kleszken, E., Purcarea, C., Pallag, A., Ranga, F., Memete, A.R., Miere, F. and Vicas, S.I., 2022. 2. Phytochemical profile and antioxidant capacity of Viscum album Subsp. album and effects on its host trees.Plants,11, 3021. 3. Melo, M.N.D.O., Batista, J.V.D.C., Peñaloza, E.M.C., Oliveira, A.P., Garrett, R., Baumgartner, S. and Holandino, C., 2023. 4. A scoping review of genus Viscum: biological and chemical aspects of alcoholic extracts.Plants,12, 1811.Nazaruk, J. and Orlikowski, P., 2016. 5. Phytochemical profile and therapeutic potential of Viscum album Natural Product Research,30(4), 373-385.Szurpnicka, A., Wrońska, A.K., Bus, K., Kozińska, A., Jabłczyńska, R., Szterk, A. and Lubelska, K., 2022. 6. Phytochemical screening and effect of Viscum album on monoamine oxidase A and B activity and serotonin, dopamine and serotonin receptor 5-HTR1A levels in Galleria mellonealla (Lepidoptera).Journal of Ethnopharmacology,298, 115604.
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