Evaluation of cell viability of (Zn)CuInS/ZnS-TPPS ₄ conjugates against various cell lines as a potential fluorescent probe Ncediwe Tsolekile 1 , Mangaka Matoetoe 1 , Sandile Songca 2 , Oluwatobi Oluwafem 3 1 Chemistry Department, Cape Peninsula University of Technology, South Africa, 2 Chemistry Department, University of Kwazulu Natal, South Africa, 3 Department of Chemical Sciences, University of Johannesburg, South Africa We present a straightforward synthesis strategy for synthesizing (Zn)CuInS/ZnS-TPPS 4 conjugates and investigate their cytotoxicity as promising fluorescent probes. The quantum dots (QDs) were produced utilizing eco-friendly materials such as glutathione and water as a solvent, whereas the 10,15,20-(4-sulphonatophenyl) porphyrin (TPPS 4 ) was produced by acidifying a meso-tetraphenylporphyrin precursor 1 . The high-water dispersion, stability, and ability to generate singlet oxygen have sparked interest in TPPS 4 . Titrating porphyrin with varying concentrations of (Zn)CuInS/ZnS QDs while holding all other experimental parameters constant resulted in the conjugation of (Zn)CuInS/ZnS QDs with TPPS 4 . Comparing the conjugate to bare QDs and porphyrin demonstrated improved spectrum and photo-physical characteristics. The MTT cell viability assay was used to compare the cytotoxicity of TPPS 4 and (Zn)CuInS/ZnS-TPPS 4 conjugates in BHK21, Hela, A549, Hek 293, and B16-F10 Nex 2 cell lines. The results revealed that the compound has little in vitro cytotoxicity, indicating that it is a good and biocompatible option for imaging and phototherapy applications. References 1. Tsolekile, N.; Parani, S.; Lebepe, T.C.; Maluleke, R.; Ncapayi, V.; Matoetoe, M.C.; Songca, S.P.; Oluwafemi, O.S. Cell Viability Study of ZnCuInS/ZnS–TPPS 4 Conjugatesagainst Different Cell Lines as a Promising Fluorescent Probe. Organics 2023, 4, 126–136.
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