Evaluation of medicinal properties of selective phytochemicals from soy and pea as anti-inflammatory agents Dr. Mala M. Parab, Maherukh S., Pramodkumar P Gupta, Debjani Dasgupta School of Biotechnology and Bioinformatics, D Y Patil Deemed to be University, India IInflammation is a complex physiological response that plays a crucial role in various diseases, including chronic inflammatory conditions. Natural compounds such as genistein found in soy and pea, have gained considerable attention due to their potential therapeutic benefits. This study aimed to evaluate the anti-inflammatory effects of soy and pea extracts containing genistein and their interaction with TNF-alpha, a key proinflammatory cytokine. The in-silico analysis revealed anti-inflammatory activity of genistein by attenuating TNF-alpha-induced inflammation. Employing in-vitro cell line model- based analysis, the extracts and genistein were observed to suppress the activation of NF-κB, a transcription factor crucial for inflammatory responses. Additionally, cell viability assays demonstrated that the treatments did not exert cytotoxic effects on the cells. The present study also highlights the significant antioxidant activity exhibited by soy and pea due to their rich content of bioactive compounds. These compounds have been shown to scavenge free radicals, reducing oxidative stress and potentially preventing various chronic diseases. References 1. Shekhar, Hossain; Uddin, Howlader; Zakir Hossain; Kabir, Yearul (July 22, 2016). Exploring the Nutrition and Health Benefits of Functional Foods. IGI Global. p. 223. ISBN 978-1-5225-0592-1. Retrieved November 22, 2017. 2. Ghulam Raza; Mohan B. Singh; Prem L. Bhalla (June 11, 2017). Atanassov, Atanas (ed.). "In Vitro Plant Regeneration from Commercial Cultivars of Soybean". BioMed Research International. 2017: 7379693. doi:10.1155/2017/7379693. PMC 5485301. PMID 28691031. 3. Purcell, Larry C.; Salmeron, Montserrat; Ashlock, Lanny (2000). "Chapter 19: Soybean Facts" (PDF). Arkansas Soybean Production Handbook – MP197. Little Rock, AR: University of Arkansas Cooperative Extension Service. p. 1. Retrieved September 5, 2016. 4. Nancy Bush (2014). The Minnesota Soybean Field Book (PDF). St Paul, MN: University of Minnesota Extension. p. 33. Archived from the original (PDF) on September 30, 2013. Retrieved September 16, 2016. 5. Koshino, Lívia L.; Gomes, Clarissa P.; Silva, Luciano P.; Eira, Mirian T.S.; Bloch Jr., Carlos; Franco, Octávio L.; Mehta, ngela (November 26, 2008). "Comparative Proteomical Analysis of Zygotic Embryo and Endosperm from Coffea arabica Seeds". J. Agric. Food Chem. 56 (22): 10922–26. doi:10.1021/jf801734m. PMID 18959416. 6. Seber, LE; Barnett, BW; McConnell, EJ; et al. (2012). "Scalable purification and characterization of the anticancer lunasin peptide from soybean". PLOS ONE. 7 (4): e35409. Bibcode:2012PLoSO...735409S. doi:10.1371/journal.pone.0035409. PMC 3326064. PMID 22514740. 7. Moses, T; Papadopoulou, K.K.; Osbourn, A (2014). "Metabolic and functional diversity of saponins, biosynthetic intermediates and semi-synthetic derivatives". Critical Reviews in Biochemistry and Molecular Biology. 49 (6): 439–62. doi:1 0.3109/10409238.2014.953628. PMC 4266039. PMID 25286183.
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