Exploring solvent effect on chemical profile and invitro anti plasmodial activity of root extracts of an under explored African plant; Triplochiton scleroxylon Yahaya Yakubu, Gabi Baba, Lateefa Mustafa Sulaiman, Zakariyya Jibril, Radiatu Jibril,
Firdausi Bello, Idris Abdullahi Kaduna State University, Nigeria
The search for plants with antimalarial potential lately is on the increase due to the resistance of the plasmodium to existing drugs. This study profiled for the first time the metabolite root extracts of Triplochiton scleroxylon using gas chromatography-mass spectrometry (GCMS), qualitative phytochemical screening and evaluated the In vitro antimalarial potential. The plant is traditionally used in some parts of Nigeria to manage and treat malaria. The GCMS was conducted using extracts from solvents of increasing polarity (n-hexane, ethyl acetate, ethanol and methanol) and the qualitative phytochemical screening was done using the reference methods on extracts obtained. The In vitro anti-plasmodia assay was conducted using the World Health Organization method [in-vitro micro-test (Mark III)] in a 96-well microtiter plate based on assessing schizont maturation inhibition. The results from the GCMS indicated the presence of some fatty acids such as oleic acid, Methyl Stearate, 9-Eicosene(E), and Linoelaidic acid in the different extracts while the qualitative phytochemical screening revealed the presence of alkaloids, terpenes, flavonoid, steroids and saponins in the various extracts. The ethanol extract inhibited the schizont maturation the most with IC 50 2.56±0.97 µg/mL. The result of this study validates the folkloric use of the plant root as an antimalarial agent and we believe further study we are currently working on will lead to the active phyto-constituent responsible for the activity and by extension setting a basis for a novel antimalarial drug. References 1. Department of Pure and Applied Chemistry, Faculty of Physical Sciences, Kaduna State University 2. Department of Biochemistry, Faculty of Life Sciences, Kaduna State University 3. Department of Pharmaceutical and Medicinal Science, Faculty of Pharmaceutical Sciences, Kaduna State University
P79
© The Author(s), 2023
Made with FlippingBook Learn more on our blog