DNA Detective: Reuniting Families with DNA Fingerprinting and Electrophoresis
Agarose Gel Electrophoresis, continued
8. PLACE the gel (on the tray) into the electrophoresis chamber. COVER the gel with 1X electrophoresis buffer (See Table B for recommended volumes). The gel should be completely submerged.
GEL LOADING TABLE
9. LOAD the entire sample (35 µL) into the well in the order indicated by the Gel Loading Table. 10. CHECK that the gel is properly ori- ented, then PLACE the safety cover onto the chamber. Remember, the DNA samples will migrate toward the positive (red) electrode. 11. CONNECT the leads to the power source and PERFORM electrophore- sis (See Table C for time and voltage guidelines).
LANE TUBE
SAMPLE
Standard Dye Markers Mother 1 Mother 2
Tube A Tube B Tube C Tube D Tube E Tube F
1 2 3 4 5 6
Boy 1 Boy 2 Father
12. After electrophoresis is complete, REMOVE the gel and casting tray from the electrophoresis chamber and PROCEED to visualization.
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