DNA Detective: Reuniting Families with DNA Fingerprinting and Electrophoresis
Pipetting by Numbers: STEAM Pipetting Practice Biotechnology & Pipetting: An Essential Tool Biotechnology is used in crime-solving, food production, medicine, and environmen- tal protection. Scientists rely on biological tools like enzymes and bacteria, as well as mechanical instruments such as microscopes and pipettes. Among these, pipettes are crucial for accurately measuring and transferring liquids in nearly all biotech labs. Types of Pipettes Pipettes range from simple Pasteur pipettes, resembling eye droppers, to more advanced micropipettes used in molecular biology. Graduated pipettes handle larger volumes, often requiring electronic pumps. Modern piston displacement micropi - pettes allow precise volume adjustments, while multichannel and robotic pipettes streamline complex procedures. Using a Micropipette
Parts of an adjustable micropipette:
Proper pipetting follows key steps: setting the volume, aspirat - ing liquid by creating a vacuum, dispensing liquid, repeating as needed, and purging the remain- ing sample. Accurate technique— such as using the correct pipette and tip, maintaining a vertical position, and avoiding cross-con- tamination—ensures experimen - tal success. Accuracy & Precision Successful experiments require both accuracy (closeness to the true value) and precision (repeat- ability). Scientists achieve this through proper pipette handling, consistent technique, and regular calibration. Mastering these skills is essential for reliable biotech- nology research.
Pipetting button
Volume-setting wheel
Tip ejection key
Volume lock Volume display
Handgrip
Volume range
Pipette shaft
Tip cone
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