DNA Detective: Reuniting Families with DNA Fingerprinting and Electrophoresis
Introduction to Micropipetting, continued
8. SLIDE the pipette and tip out of the tube using the inside wall to dislodge any excess droplets that may be adhering to the outside of the tip. Then MOVE your pipette to just above the first practice circle. 9. Slowly DEPRESS the plunger to the first stop. You should see the liquid flowing out of the pipette tip. 10. Gently TOUCH the tip to the paper to create a capillary effect that will help draw any remaining fluid out of the tip. 11. Keeping the tip on the paper, DEPRESS the pipette plunger to the second hard stop to ensure all liquid has been ejected. 12. RAISE the pipette so that the tip is no longer on the paper and then RELEASE the plunger to its original position. (This is more important when pipetting from one liquid solution into another but also good practice here.) 13. EJECT the tip into a beaker for used tips. 14. REPEAT Steps 2-13 for the remaining circles. As this is practice, you may want to experiment and observe what happens when common micropipetting errors are made. For instance, you could intentionally press down to the second stop while sucking up the liquid or intentionally skip pressing down to second stop when ejecting the liquid to see how this affects circle size.
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