DNA Detective: Reuniting Families with DNA Fingerprinting and Electrophoresis
Pipetting By Numbers
1. OBTAIN a segment of the Pipetting By Numbers canvas. Each segment will have a slightly different pattern. 2. NOTE that colors and pipetting volumes are indicated inside each circle. Color abbreviations are Red (R), Blue (B), Purple (P), Yellow (Y), and Navy (N). Volumes are given in microliters. So a circle marked "R20" would get 20 µL of the red dye. 3. USE the techniques you just practiced from pages 13-14 to pipette the specified Use a small amount of tape to secure the top and bottom corners of the can- vas to your lab bench. Fasten the tape to the back side (bench facing side) by wrapping the tape into a cylinder. • Hold the pipette perpendicular to the paper & pipette into the center of each circle. • When possible, start with the largest circles / highest volumes. • To save time, pipette all the same colored dots at once using the same tip. Then switch tips and move to the next color. • Dyes vary in color and in viscosity (thickness). High viscosity dyes like the Navy and Red will require slower pipetting. 4. COMBINE your finished art with other groups by matching the edge patterns to create a single poster. color and volume. For Best Results: •
Featured Experiment
Cat. #S-45 Pipetting By Numbers: STEAM Pipetting Practice For 10 Lab Groups. Help your students master the fundamental biotechnology technique of micropipetting while creating a colorful, science-themed classroom poster as well as their own artwork. • Set your classroom up for a year of experi- mental success by mastering micropipetting • Learn best practices, key parts, and the impor- tance of accuracy & precision • Engage students with a STEAM wet lab based on “paint by numbers” • Create a colorful, science-themed classroom poster
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