Color Your Classroom: Engaging Students with Bacteria and Bio-Art
Experiment Results and Analysis
-DNA plated with control cells (no DNA) Result: No colored cells visible. White colonies. May look like a smeared layer of cells. Demonstrates: Host bacterial cells are viable in the absence of ampicillin.
-DNA/+Amp plated with control cells (no DNA)
+DNA/+Amp/+IPTG plated with transformed
+DNA/+Amp plated with transformed cells (Rainbow Trans- formation Mixture)
cells (Rainbow Transformation Mixture)
Result: No colonies visible.
Result: White colonies.
Result: Individual pink, blue, and purple colonies. Demonstrates: Cells are resistant to Ampicillin when transformed with the mixture of three plasmids (pChromoBlue, pChromoPink, pChromoPurple). Production of chromogenic protein is turned on in the presence of IPTG.
Demonstrates: Untransformed cells are sensitive to ampicillin.
Demonstrates: Cells are resistant to Ampicillin when transformed with the mixture of three plasmids (pChromoBlue, pChromoPink, pChromoPurple). The colored proteins are not produced in the absence of IPTG.
Incorporating STEAM into Your Transformation Experiment Art, design, and innovation are key components to a balanced science education. Incorporating Art into your STEM curriculum can be a fun way to engage students and build a truly integrated curriculum. In this simple STEAM experiment the bacteria previously transformed by your students will become living paint for their artwork. All You Need: • ReadyPour™ Luria Broth Agar Base with Ampicillin Cat. #616 • Large Petri Plates Cat. #643 • IPTG Cat. #613 • Luria Broth Media Cat. #611 • Sterile Loops Cat. #667 Brief Experimental Procedure Each student or student group will share the transformed bacterial plate. 1. Using a sterile loop, scrape bacteria off of the transformation plate and resuspend in 1 mL of LB or Recovery Broth in a snaptop tube. 2. Using the same loop, spread the resuspended bacteria onto a fresh LB/Amp/IPTG plate.
NOTE: Encourage students to sketch their design before streaking onto their plates.
3. Place the plates in an inverted position in a 37°C incubator overnight before viewing the results.
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