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Trailblazers: Investigating Chemotaxis with C.elegans

Module III: Collection of C.elegans and Chemotaxis Assay Excerpts from Edvo-Kit #852

12. ADD 40 µL of the wild-type worms to center hole of the wild-type plate. With a new small trans- fer pipet or pipet tip REPEAT for the mutant strain. 13. Allow the plates to INCUBATE face up on your bench at room temperature for 15 minutes. 14. OBSERVE the movement of the worms under the microscope and count the number of worms in the Experimental (left) and Control (right) areas. RECORD these numbers in Table 2. NOTE: By 15 minutes, worms should be visible and moving across the plate surface. If there are no worms on the NGM agar, examine the central well. Surface tension may be keeping the worms caught within this well. If so, carefully use a kimwipe to touch the edge of the drop and wick away excess liquid. 15. (Optional) CREATE a time series by repeating step 13 and 14 two more times. 16. INCUBATE worms overnight.

Table 2

Wild-Type

Mutant Strain

# of Worms in Experimental Area

# of Worms in Control Area

# of Worms in Experimental Area

# of Worms in Control Area

15 min.

15 min.

30 min.

30 min.

45 min.

45 min.

9

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