"-""'. m UNIVERSITEIT GENT
LABO VOOR MICROBIËLE ECOLOGIE EN TECHNOLOGIE
(LabMET) PROF. DR. IR. W. VERSTRAETE
Ghent, 12thJune 2006
To Mr. CorrieGielen - Chrisal NV Priester Daensstraat 9 3920 Lommel
IReport 0" microbial tests towards dust mitel
In the framework of Chrisal's R&D strategy for the Probiotics In Progress (PIP) product line, LabMET performed a number of microbial tests to verify the repellent activity of sporulating bacteria towards dust mite. Three types of experiments were performed in triplicate and are presented below.
1) Repellent action of PIP product towards dust mite
Sealed and aerated plastic containers, provided with a textile bottom, were inoculated with dust mite and incubated at 28°C at a humidity of 70%. No additional feed was added. Treatment of the containers with Chrisal's PIP product was performed on the right half of the textile surface, with the other half being treated with an equal volume of sterile water. Treatment protocols were as follows :
Container 1 : Control (Ieft and right side treated with water) Container 2 : Daily treatment of the right side with PIP product (during 8 days) Container 3 : 72 hours treatment of the right side with PIP product (during 2 weeks) Container 4 : Weekly treatment of the right side with PIP product (during 3 weeks)
Container 1 : Data are averaged over three replicate experiments. No significant differences were obtained between the dust mite counts on both halves of the surface. This indicates that with no treatment, the dust mites migrate randomly across the surface. The slight rise in absolute dust mite numbers also indicatesthat the lack of supplementedfood is not detrimentalto the population.
Fig 1 presents the results on dust mite counts of container 1.
UNIVERSITEIT GENT - FACULTEIT BIO-INGENIEURSWETENSCHAPPEN
- LabMET -
9000 GENT - BELGIË
Dr. Robin Temmerman- Project manager TEL +32(0)9 2645976 - FAX +32(0)9 2646248 - EMAIL Robin.Temmerman@UGent.be http://labmet.ugent.be
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