01 - Left at the Scene of the Crime: Intro to Forensic Science
Experiment Procedures: Agarose Gel Electrophoresis
9. PLACE gel (on the tray) into electrophoresis chamber. COVER the gel with 1X electrophoresis buffer (See Table B for recommended volumes). The gel should be completely submerged. 10. LOAD 25 µL of each QuickStrip™ sample into the well in the order indicated by Table D. 11. PLACE safety cover. CHECK that the gel is properly orient-
Reminder: Before loading the samples, make sure the gel is properly oriented in the ap- paratus chamber.
ed. Remember, the DNA samples will migrate toward the positive (red) electrode. source and PERFORM electropho- resis (See Table C for time and volt- age guidelines). 13. After electrophoresis is complete, REMOVE the gel and casting tray from the electrophoresis chamber. 12. CONNECT leads to the power
LANE LABEL
SAMPLE NAME
1 2 3 4 5 6
A B C D E --
DNA Standard Marker Crime Scene PCR reaction Suspect 1 PCR reaction Suspect 2 PCR reaction Suspect 3 PCR reaction
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Time and Voltage Guidelines (0.8% Agarose Gel)
1x Electrophoresis Buffer (Chamber Buffer)
EDGE™
M12 & M36
Volts
Min/Max (minutes)
Min/Max (minutes)
EDGE™
150 mL
3 mL
147 mL
150 125 100
10/20
20/35 30/45 40/60
M12
400 mL
8 mL
392 mL
N/A
M36
1000 mL
20 mL
980 mL
15/25
9
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