Monoclonal Antibodies (mAbs) Testing services 25 10

Monoclonal Antibodies (mAbs) Testing Services

Health Inspired, Quality Driven.

Testing along the mAb process

Engage SGS experts early in your monoclonal antibody program. From discovery through

PK/PD BIOANALYSIS

CELL LINE GENERATION

PROCESS OPTIMIZATION

ENGINEERING RUN

GMP MANUFACTURING

FORMULATION (FILL & FINISH)

CLINICAL STUDIES

monoclonal antibody development process

to market entry, we simplify complexity,

minimize technical and communication barriers, and significantly accelerate regulatory approval.

Our clients can rely on SGS global analytical capabilities and dedicated expertise to accelerate their mAbs project

CELL BANK TESTING • Microbiological • Identity • Viral safety testing • Genetic stability

ANALYTICAL DEVELOPMENT • Methods to monitor critical Quality Attributes

ANALYTICAL DEVELOPMENT • Methods in line with ICH Q14

TRIAL PHASES I, II & III • Biomarker development • Validation of bioanalytical methods

GMP VALIDATION • Methods in line with ICH Q2(R2)

STABILITY TRIALS • Drug Substance • Drug Product

BIOSAFETY

In vitro adventitious virus assays • Endotoxins •

• TEM • Retroviruses detection assays

Mycoplasma (culture + qPCR) • Sterility / Bioburden

Testing for CHO biologics

CHO uBH Characterization Accelerated

CHO uBH Characterization Conventional

Sterility direct inoculation (USP <71>, E.P. 2.6.1)

Sterility direct inoculation (USP <71>, E.P. 2.6.1)

Mycoplasma qPCR

Mycoplasma (USP<63>,E.P. 2.6.7)

28day in vitro (CPE/HAD/HA) 14day assay time based on risk

Risk based panel of degenerate qPCRs

Detection of MVM by qPCR

Detection of MVM by qPCR

Detection and quantitation of C-Type RVLPs by qPCR

Virus particle detection and quantitation by thin section electron microscopy

Biosafety testing of CHO biologics

• Rapid targeted NAT - a fast, ICH Q2-validated alternative to traditional in-vitro assays, accelerating CHO-based biologics manufacturing • Covers 39 qPCR targets across 15+ high-risk viral families (Hsu et al., 2024) for comprehensive detection • Uses TaqMan® chemistry with degenerate primers/probes for broad and specific viral identification, contamination-free and highly sensitive

• Fully aligned with ICH Q5A(R2) guidelines; NGS options available for in-vivo replacement and cell bank testing • Risk-based panel design enabling faster, confident batch release and shorter time to patient

Expert guidance for your analytical strategy

Identify attributes • Identification of attributes

Validate methods • In line with ICH Q2(R2) validation of methods applied to critical Quality Attributes (batch release, stability studies) Trending • Monitoring of method performance • Monitoring of product attributes along its lifecycle

• Product knowledge • Forced degradation Develop method • Adequate selection of technology and instrument • Proof of concept study to confirm methodology Fit-for-purpose • Assessment of analytical procedure performance • Documentation of analytical procedure (including control strategy)

Why SGS: Complete mAbs Project Support

QC & stability (DS, DP, raw materials)

Characterization

Biosafety

Bioanalysis and clinical

STRUCTURAL CHARACTERIZATION • Composition, sequencing, PTMs HIGHER ORDER STRUCTURE • Secondary, tertiary and aggregates FUNCTIONAL CHARACTERIZATION • Ligand binding and cell-based technologies IMPURITY IDENTIFICATION • Product- & process-related impurities

RELEASE TESTING • Compendial, bespoke ID tests • Potency and function MICROBIOLOGY

V IRUS DETECTION ASSAYS • In-vitro, qPCR, TEM CELL IDENTITY & CHARACTERIZATION • DNA fingerprinting, Sanger sequencing GENETIC STABILITY • Southern blot, DNA sequencing (NGS)

ACROSS DRUG DEVELOPMENT PROCESS • From discovery pharmacology to late clinical stages REGULATORY TOXICOLOGY PRECLINICAL AND CLINICAL • Pharmacokinetics (PK) • Immunogenicity (ADA, Nab) • Pharmacodynamics (Cellular/Soluble Biomarkers)

• Sterility, bioburden, endotoxin QUANTITATIVE IMPURITY ANALYSES • Product- and process-related impurities STABILITY TESTING • Accelerated, standard

Expertise for mAbs Producers

SGS Laboratories

Method development, transfer and validation

Biosafety testing

Batch release testing

Characterization studies

+200 TESTS PER DRUG PER YEAR >95% ON TIME DELIVERY

>1200 PHYSICOCHEMICAL TESTS PER YEAR >200 BATCHES TESTED PER YEAR

<4 WEEKS FAST-TRACK METHOD TRANSFER OF EIGHT BIOASSAYS

>50 CHARACTERIZATION PROJECTS TO US FDA & EMA GUIDELINES

Highest quality GMP biosafety testing facility with 72 segregated BSL-2 labs

Our routine batch release and stability studies include physicochemical, potency, purity, microbiological tests

We are experienced in developing complex assays and fast-tracking method transfer

We applied ICH Q6B across diverse studies from insulin to complex biotherapeutic modalities, ensuring compliance with biologics guidelines

Expertise for mAbs Producers

SGS Bioanalysis / Bioequivalence Laboratories

Lab capabilities

Expertise & technologies

Regulatory compliance

5 LABORATORIES (EUROPE, NORTH AMERICA, CHINA) > 600 Projects conducted per year > 2500 Assays developed & validated > 300k Samples analyzed per year > 250 Experts > 35 Years of experience

ALL MODALITIES (NCE, NBE, ADCS, BIOSIMILARS, CGT)

GXP & CFR PART 11 COMPLIANT

• Immuno Assays: Spectramax, MSD, Luminex, Ella™,Cobas, Gyrolab, Wizard • Cell-Based Assays & Molecular Biology: Flow Cytometry, qPCR • LC-MS/MS & LC-UV-Vis/Fluorescence: Sciex, Waters, Shimadzu • Robotic: TECAN & TECAN EVOLizer (TECAN), Andrew (Waters), Extrahera (Biotage)

• US Food and Drug Administration (FDA) • European Medicines Agency (EMA) • UK Medicines and Healthcare products Regulatory Agency (MHRA) • Japan Pharmaceuticals and Medical Devices Agency (PMDA)

Expertise for mAbs Producers

SGS Clinical Pharmacology Unit

Clinical pharmacology unit

Biometrics

Regulatory

Clinical operations

110-BED PURPOSEBUILT EARLY PHASE UNIT

400+ EXPERTS IN DATA MANAGEMENT, BIOSTATISTICS & PK/PD DATA ANALYSIS

SUPPORT FOR CLINICAL TRIAL SUBMISSION & EMA / FDA APPROVALS

GLOBAL CLINICAL RESEARCH / PROJECT MANAGEMENT SERVICES

Extensive experience in biologics and first-in-human trials On-site GMP production facility with 2 QPs

High quality CDISC compliant datasets Ongoing interactive data visualizations

Fastest EU approval timelines for biologic products

Local and international partnerships for patient cohort or ethnic bridging

Monoclonal Antibodies (mAbs) Testing Services

Health Inspired, Quality Driven.

Contact us

pharma@sgs.com sgs.com/pharma sgs.com/linkedinpharma

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