Putting enzymes on pause
(a)
(b)
Figure 2: Scatterplot graphs depicting time versus pNP absorption data for the time course assay for enzyme concentrations of 0.1, 0.3, 1, 3, and 7.905 μM of concentration for a 30 -minute assay (a) and the pNP absorption data for 0.3 and 1 μM enzyme concentration fo r the first 5 minutes of the assay (b). Linear regression analysis was performed, yielding slope equations and R² values, which are depicted on the chart. Running the steady-state enzyme assay, measuring the rate of pNP produced by Cdc14, and graphing the values as a function of time elapsed versus the absorbance values of pNP resulted in data demonstrating the linear range for each enzyme concentration (Figure 2a). Further examination of the linear ranges of the catalysed reaction at enzyme conce ntrations of 0.3 μM and 1 μM (Figure 2b) provided information regarding the enzyme concentration that had the most consistently linear increase in pNP absorbance over 5 minutes.
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