Putting enzymes on hold
synthesized and tested in vitro on Cdc14 to prove that it effectively binds and functions as an inhibitor. Secondly, a toxicity assay should be conducted on the designed inhibitor in order to confirm that it is non-toxic. Thirdly, the designed inhibitor must be tested in vivo on A. alternata to prove that it can effectively penetrate the fungal cell membrane and reach the area adjacent to the nucleus. If this is not possible, then another system must be designed, or the inhibitor must be modified. Finally, the inhibitor must be tested on non-target organisms, including crops and animals (and later humans), to prove that it does not have harmful effects. Nevertheless, the designed competitive Cdc14 inhibitor is a candidate for future drug development. Moreover, due to the conserved nature of Cdc14 across many fungi, this investigation now serves as the groundwork for future in-depth studies into not only targeted A. alternata fungicides but also pesticides for a host of other fungal pathogens, such as Rhynchosporium commune , Fusarium oxysporum , and Penicillium digitatum .
Bibliography
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