ICCFGG 2022
#63 Whole-genome shotgun metagenomic sequencing reveals distinct gut microbiome signatures of obese cats Xu Wang1,3,7,8 , Xiaolei Ma1,2, Emily Brinker1, Emily C. Graff1,3, Wenqi Cao1, Amanda L. Gross3, Aime K. Johnson4, Chao Zhang5, and Douglas R. Martin3,6 xzw0070@auburn.edu 1Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA, 2School of Life Sciences and Technology, Tongji University, Shanghai, China, 3Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, AL, USA, 4Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL, USA, 5Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Institute of Precision Medicine, Shanghai JiaoTong University School of Medicine, Shanghai, China, 6Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA, 7Center for Advanced Science, Innovation and Commerce, Alabama Agricultural Experiment Station, Auburn, AL, USA, 8HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA Overweight and obesity are growing health problems in domestic cats, increasing the risks of insulin resistance, lipid dyscrasias, neoplasia, cardiovascular disease, and decreasing longevity. The signature of obesity in feline gut microbiota has not been studied at the whole-genome shotgun (WGS) metagenomic level. We performed WGS metagenomic sequencing in the fecal samples of 8 overweight/obese and 8 normal cats housed in the same environment. We obtained 271 Gb sequences and generated a 961 Mb de novo reference contig assembly, with 1.1 million annotated microbial genes. In the obese cat microbiome, we discovered a significant reduction in microbial diversity and Firmicutes abundance, as well as decreased Firmicutes/Bacteroidetes ratios, which is the inverse of obese human/mouse microbiota. Linear discriminant analysis and qPCR validation revealed significant increases in Bifidobacterium sp., Olsenella provencensis, Dialister sp.CAG:486, and Campylobacter upsaliensis as the hallmark of obese microbiota among 400 enriched species, whereas 1,525 bacterial species have decreased abundance in the obese microbiome. Phascolarctobacterium succinatutens and an uncharacterized Erysipelotrichaceae bacterium are highly abundant in the normal gut with over 400-fold depletion in the obese microbiome. Fatty acid synthesis-related pathways are significantly overrepresented in the obese compared to the normal cat microbiome. In conclusion, we discovered a decreased microbial diversity in obese cat gut microbiota, suggesting potential dysbiosis. A panel of 7 significantly-altered, highly-abundant species can serve as a microbiome indicator of obesity. Our findings in the obese cat microbiome composition, abundance, and functional capacities provide new insights into feline obesity.
#64 Transcriptome landscape of the canine zonary placenta Yue Zhang yzz0208@auburn.edu Auburn University, Auburn, AL, USA
The placenta is a critical organ connecting the mother with the developing conceptus in utero. Despite its critical functions, the placenta is the most morphologically diverse organ in mammals. The dog has a unique zonary placenta, which is a belt-shaped structure surrounding the fetus. Characteristic structures include the transfer zone (TZ) and pigmented zone (PZ), which are unique features compared to other placental forms. However, the functions and gene expression
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