ICCFGG 2022
#1 Comparative analysis of single haplotype genomes unmasks the role of structural variation in felid evolutionary innovation William J. Murphy 1,2 *, Kevin R. Bredemeyer 1,2 , LaDeana Hillier 3 , Andrew J. Harris 1,2 , Graham Hughes 4 , Edward Rice 5 , Terje Raudsepp 1,2 , Brian W. Davis 1,2 , Stephen J. O’Brien 6,7 , Leslie A. Lyons 8 , Wesley C. Warren 5 wmurphy@cvm.tamu.edu 1 Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA, 2 Interdisciplinary Program in Genetics & Genomics, Texas A&M University, College Station, TX, USA, 3 Department of Genome Sciences, University of Washington, Seattle, WA, USA, 4 Department of Zoology, University College Dublin, Dublin, Ireland, 5 Department of Animal Science and Bond Life Science Center, University of Missouri, Columbia, MO, USA, 6 Theodosius Dobzhansky Center for Genome Bioinformatics, Saint- Petersburg State University, Saint-Petersburg, Russia, 7 Oceanographic Center, Nova Southeastern University, Fort Lauderdale, FL, USA, 8 Department of Veterinary Medicine & Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO, USA The role of structural variation in speciation is poorly understood. The recent divergence of the cat family, Felidae, provides a tractable system to study genomic and phenotypic divergence in unprecedented detail using near-gapless genome assemblies derived from F1 interspecies hybrids. Here we describe highlights from a comparative analysis of six single haplotype genome assemblies sampled across the felid phylogeny, where greater than 99.5% of the euchromatic sequence is assembled into chromosomes. Felid genomes are highly colinear, with a paucity of autosomal chromosome rearrangements, and enrichment of microinversions on the X chromosome. We observed rapid rates of sequence evolution across large, functional X-linked satellite repeats with known roles in X chromosome inactivation and felid speciation. High resolution maps of olfactory receptor gene family dynamics reveal surprising functional variation that we interpret in the context of domestication and life history traits. Finally, we present the first insights into the structure of cat centromeres and illustrate parallels and differences with their primate counterparts.
#2 Genetics of chronic superficial keratitis in the Australian Racing Greyhound
Claire M Wade 3 * , Steven Karamatic 1 , Rebecca Goode 2 , Niruba Bageswaran 3 , Cali E Willet 4 , Georgina Samaha 4 , Ray Ferguson 5 , Hamutal Mazrier 6 claire.wade@sydney.edu.au 1 Greyhound Racing Victoria, West Melbourne, VIC 3003 AUSTRALIA; 2 Greyhound Adoption Program, Greyhound Racing Victoria, Seymour VIC 3660 AUSTRALIA; 3 Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Camperdown, NSW 2006 AUSTRALIA; 4 Sydney Informatics Hub, The University of Sydney, Camperdown, NSW 2006, AUSTRALIA; 5 Australian Greyhound Working and Sporting Dog Veterinarians, East Oakleigh Vic. 3166 AUSTRALIA; 6 Faculty of Science, Sydney School of Veterinary Sciences, The University of Sydney, Camperdown, NSW, 2006 AUSTRALIA
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