PRESENTATION ABSTRACTS
consortium should push the next dataset to close to 500 cats. The bioinformatics team at MU will be collating the cat genomes, both private and public data, to produce a new combined variant call file for the cat research community. Members of the consortium have discussed criteria for data inclusion, workflows and the base and variant quality score recalibration steps. Additional WGS data from the closest outgroup species, the African wildcat (Felis lybica) and European wildcats (F. silvestris), will be part of upcoming inclusions in the 99 Lives project. The success of variant discovery for traits and diseases is ~50% and will be highlighted. #32 Speed versus endurance; the biological implications of the selection of sprint and distance Alaskan Sled dogs Huson, H.J. 1 , Srikanth, K. 1 , Thorsrud, J.A. 1 , Ostrander, E.A. 2 , Rundstadler, J.A. 3 hjh3@cornell.edu 1 Cornell University, College of Agriculture and Life Sciences, Department of Animal Science, Ithaca, NY, USA, 2 National Institutes of Health, Human Genome Research Institute, Cancer Genetics and Compara- tive Genomics Branch, Bethesda, MD, USA, 3 Tufts University, Cummings School of Veterinary Medicine, Department of Infectious Disease and Global Health, N. Grafton, MA, USA Alaskan sled dogs (ASD) are well known for their endurance, traversing over 1,000 miles of harsh arctic terrain in extreme temperatures. Endurance dogs completing the Iditarod Sled Dog Race average approximately 8 mph with the fastest teams covering the 1,000 miles in 8 days. Less known to the public are sprinting ASD who race heats of 4-30 miles over 2-3 consecutive days. Sprint dogs can exceed speeds of 20 mph. In comparison to many registered breed dogs, ASD are selected solely for performance and have mixed ancestry including a foundation similar to arctic breeds such as the Siberian Husky and Alaskan Malamute. Furthermore, lineages of ASD have been dif- ferentially selected within the population for sprint or distance racing for nearly a century creating genetic substructure within ASD. This study compared the genome of sprint and distance dogs to identify signatures of selection differentiating dogs within the two racing types. Illumina ® Canine HD arrays with 170-220K SNPs were used to genotype 328 sprint and 222 distance ASD. Runs of homozygosity (ROH) were identified and a numeric association study performed to identify ROH differentially represented in racing type. Genes within associated ROH were analyzed for overrepre- sentation compared to the genome using the software PANTHER to assess biological significance. Biological pathways including multiple metabolic processes, respiratory electron transport chain, locomotion, muscle contraction and development, blood circulation, and metabolite and energy precursors were significantly associated (Bonferroni corrected p-value<0.05) and provide initial insight into the biological implications of ASD selection for speed or endurance.
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