POSTER ABSTRACTS
#20 Genetics of vaccine-induced immune response in Beagles Jeanna M. Blake1 , James Thompson2, Dayna L. Dreger3, Harm HogenEsch4, Kari J. Ekenstedt1 gwigley@purdue.edu 1Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA, 2Zoetis, Veterinary Medicine Research and Development, Kalamazoo, MI, USA, 3National Human Genome Research Institute, NIH, Bethesda, MD, USA (Dreger), 4Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA Both genetic and non-genetic factors give rise to individual variation in the immune response to vaccination. Identifying how genetic background influences variation in both magnitude and per- sistence of vaccine-induced immunity is vital for improving vaccine development and understanding possible sources of vaccine failure. The objectives of this study were to estimate the heritability of antibody response to vaccination against viral and bacterial pathogens and to identify genes driving variation of the immune response to vaccination in Beagles. Sixty puppies were immunized following a standard vaccination schedule on days 0, 21, and 42 with an attenuated combina- tion vaccine containing antigens for canine adenovirus type 2, canine distemper virus, canine parainfluenza virus, canine parvovirus, and four strains of Leptospira bacteria. Serum antibody measurements for each viral and bacterial component were measured on days 21, 42, 56, and 63. Serum antibody levels for BSA and fibronectin, common vaccine formulation by-products, were measured on days 25, 42, 56, and 63. Heritability estimations and GWAS were conducted using Axiom CanineHD SNP array genotypes (274,898 markers), and serum antibody titers from days 21, 25, and 63. Heritability estimates were: 1) to Leptospira antigens, ranging from 0.179 to 0.697; 2) to viral antigens, ranging from 0.039 to 0.589; and 3) to vaccine formulation by-products, ranging from 0.187 to 0.441. GWAS identified two genetic markers associated with fold-change between time points for all ten phenotypes. These findings indicate that genetic regulation of the immune response to vaccination is antigen specific and influenced by multiple genes of small effect.
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