PAPERmaking! Vol3 Nr2 2017

!;1;bˆ;7Ĺƒ-m†-u‹ƑƏƐƕՊ | Պ !;ˆbv;7ĹƐƒ-u1_ƑƏƐƕՊ | Պ 11;r|;7ĹƑƐ-u1_ƑƏƐƕ DOI: 10.1002/mbo3.487

ORIGINAL RESEARCH

Characterizing bacterial communities in paper production— troublemakers revealed

Anita Zumsteg Պ | Պ"blomĺ&u‰‹Ѵ;uՊ | Պo-1_blѴ-†0b|Œ

l‹-m|;um-|bom-Ѵķ!ş b1uo0boѴo]‹ķ =|ubm];mķ"‰b|Œ;uѴ-m7

Abstract Biofilm formation is a major cause of reduced paper quality and increased down time during paper manufacturing. This study uses Illumina next-generation sequencing to identify the microbial populations causing quality issues due to their presence in bio- films and slimes. The paper defects investigated contained traces of the films and/or vѴbl;o=l-bmѴ‹|‰o];m;u-ķ Tepidimonas and Chryseobacterium . The Tepidimonas spp. found contributed on average 68% to the total bacterial population. Both genera have been described previously to be associated with biofilms in paper mills. There was in- dication that Tepidimonas spp. were present as compact biofilm in the head box of one paper machine and was filtered out by the paper web during production. On the other hand Tepidimonas spp. were also present to a large extent in the press and white wa- |;uvo=|‰omomruo0Ѵ;l-|b1r-r;ul-1_bm;vĺ$_;u;=ou;ķ|_;l;u;ru;v;m1;o=-hmo‰m biofilm producer alone is not sufficient to cause slimes and therefore paper defects -m7o|_;u1ub|b1-Ѵ=-1|ouv-u;-77b|bom-ѴѴ‹-|rѴ-‹ĺ oubmv|-m1;ķ‰;b7;m|b=b;7 Acidovorax vrĺķ‰_b1_bv-m;-uѴ‹1oѴombŒ;uo=r-r;ul-1_bm;vķ;Š_b0b|bm]|_;-0bѴb|‹|o=oul;Š|u- - 1;ѴѴ†Ѵ-u l-|ub1;v=ou-||-1_l;m|-m70bo=bѴl=oul-|bomĺ

Correspondence mb|-,†lv|;]ķl‹-m|;um-|bom-Ѵķ =|ubm];mķ"‰b|Œ;uѴ-m7ĺ l-bѴĹ-mb|-匆lv|;]Šol‹-ĺ1ol

 +)! " 0bo=bѴlvķ7bˆ;uvb|‹ķbm7b1-|ouvķl;|-];molb1vķlb1uo0b-Ѵ1om|-lbm-|bom

ƐՊ | Պ$! &$

Various bacterial species may be responsible for biofilm formation in paper machines . Deinococcus geothermalis bv-rubl-u‹1oѴombŒ;uѴ;-7 - bm] |o |_b1hķ v‹m;u]bv|b1 0bo=bѴlv‰b|_ 7b==;u;m| 0-1bѴѴb vr;1b;v ŐoѴ-ubķ ††|bm;mķş"-Ѵhbmof-Ŋ"-Ѵom;mķƑƏƏƐőĺ †u|_;ulou;ķ Tepidimonas vrrĺķ 0;Ѵom]bm] |o |_; ;|-ruo|;o0-1|;ub-ķ ‰;u; b7;m|b=b;7 7bu;1|Ѵ‹ bm |_; r-r;uruo1;vv-Ѵu;-7‹-||_;;-uѴ‹v|-];o=0bo=bѴl=oul-|bomŐ$bbuoѴ-ķ -_|bm;mķ(†;m|oķşh;uŊ ѴolķƑƏƏƖőĺ";ˆ;u-Ѵ0-1|;ub-Ѵ1Ѵ-vv;v-m7 genera are known to populate the waters and raw products in paper l-1_bm;vĺ(-bv-m;m;|-ѴĺŐƐƖƖѶő-m-Ѵ‹Œ;7ƒƖƏ1†Ѵ|bˆ-0Ѵ;-;uo0b10-1 - teria from process steps and raw materials and demonstrated a vast 0-1|;ub-Ѵ7bˆ;uvb|‹ĺ|_ouo†]_r_‹Ѵo];m;|b1-m-Ѵ‹vbvo=ƓƏƓ1Ѵom;7Ɛѵ" u! ];m; -lrѴb1omv‰-v r;u=oul;7 0‹ u-m_-ѴѴ ;|-Ѵĺ ŐƑƏƐƏőķ‰_o -m-Ѵ‹Œ;7|‰o7b==;u;m|r-r;ulbѴѴv|_-|v_o‰;7vblbѴ-uoˆ;u-ѴѴruo=bѴ;v but still unique individual populations. Bacteroidetes (including the

-r;ul-m†=-1|†ubm]u;t†bu;v-Ѵ-u];ˆoѴ†l;o=‰-|;uķ‰_b1_ķ|o7-‹ķ is permanently recycled at the various stages during the production ruo1;vvĺvv†1_ķ0-1|;ub-Ѵ]uo‰|_-m70bo=bѴl=oul-|bombm|_;r-r;u machines are inevitable. These recycled waters are a main cause of slime production related to the presence of bacteria which leads to vl;ѴѴķ7bv1oѴou-|bomķ-m7buu;]†Ѵ-ub|b;vbm|_;r-r;u=oul-|bom-m7‰;0 0u;-hvŐѴ-m1oķ;]uoķ-vr-uķş$bf;uoķƐƖƖѵĸoѴ-ubķƑƏƏƕőĺ$olb| - igate these effects the microbial population is continuously treated ‰b|_0bo1b7;vŐѴ-m1oķ;]uoķom|;ķ †;m|;ķş$bf;uoķƑƏƏƓőĺ†| ‰_;m 0-1|;ub-Ѵ 1oѴombŒ-|bom bv o†| o= 1om|uoѴķ |_; 1omv;t†;m1;v -u; ˆ-ub-0Ѵ;r-r;ut†-Ѵb|‹ķbm1u;-vbm]7o‰m|bl;ķ-m7_b]_;ul-bm|;m-m1; 1ov|vŐoѴ-ub톆|bm;mķ!-bm;‹ķş"-Ѵhbmof-Ŋ" -Ѵom;mķƑƏƏƒőĺ

$_bvbv-mor;m-11;vv-u|b1Ѵ;†m7;u|_;|;ulvo=|_;u;-|bˆ;ollomv||ub0†|bomb1;mv;ķ‰_b1_r;ulb|v†v;ķ7bv|ub0†|bom-m7u;ruo7†1|bombm-m‹l;7b†lķ provided the original work is properly cited. šƑƏƐƕ$_;†|_ouvĺ MicrobiologyOpen r†0Ѵbv_;70‹o_m)bѴ;‹ş"omv|7ĺ

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MicrobiologyOpen. 2017;6:e487.  https://doi.org/10.1002/mbo3.487



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