Investigating the pharmaceutical potential of brown rot basidiomycetes Suhad Al-Salihi 1,3 , Trong Tuan Dao 2 , Andy M. Bailey 3 , Gary D. Foster 3 1 University of Technology, Iraq, 2 School of Chemistry, University of Bristol, UK, 3 School of Biological Sciences, University of Bristol, UK There is an increasing interest in utilizing fungal secondary metabolites in the battle against emerging superbugs 1 . G. trabeum is a wood decay basidiomycete that established compact network with its environment 2 . However, information on its secondary metabolites synthesis is scare. In our study, we investigated G. trabeum antagonistic towards Escherichia coli, Saccharomyces services and Bacillus subtilis, highlighting its ability to produce antimicrobial agents. We further examined G. trabeum sensitivity against two antibiotics; glufosinate and hygromycin B, to find that hygromycin, is a reliable and cost-effective selectable marker for genetic manipulation in G. trabeum. We then chemically analyzed G. trabeum fermentation culture. This leads to the characterization of four previously undescribed rare coumarin based polyketides (Gt-1= oospoglycol, Gt-2= oosponol, Gt-3= FD48 and Gt-4), of which, Gt-2 showed antimicrobial activity against B. subtilis. G. trabeum whole genome sequences, represents the only available genomic data from the Gloeophyllales 3 , characterizing these rare compounds in this species, would provide further support towards the quest for high yield and fast production of novel antibiotics via synthetic biology platform. References 1. Al-Salihi, S.A., Scott, T.A., Bailey, A.M. and Foster, G.D. (2017). Improved vectors for Agrobacterium mediated genetic manipulation of Hypholoma spp . and other homobasidiomycetes. Journal of Microbiological Methods , 142 , pp.4-9. 2. Vesentini, D., Dickinson, D.J. and Murphy, R.J. (2005). The production of extracellular mucilaginous material (ECMM) in two wood-rotting basidiomycetes is affected by growth conditions. Mycologia , 97 (6), pp.1163-1170. 3. Floudas, D., Binder, M., Riley, R., Barry, K., Blanchette, R.A., Henrissat, B., Martínez, A.T., Otillar, R., Spatafora, J.W., Yadav, J.S. and Aerts, A. (2012). The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes. Science , 336 (6089), pp.1715-1719.
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