Galectin-3 synergizes with CD47 to Suppress Phagocytosis an…

Galectin-3 synergizes with CD47 to Suppress Phagocytosis and T cell immunity in Peritoneal Metastases of Gastric Adenocarcinoma A B C

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CD47

SIRPA

-0.5

3

Z-Score

A . Bulk RNA-seq analysis on Peritoneal metastasis (PC) specimen using a curated panel of immune checkpoint and “don’t eat me” gene. B . Bulk RNA-seq analysis on Stage IV of TCGA STAD (n=41) cohort using a notable panel of immune checkpoint and “don’t eat me” gene. C . Expression profile of “don’t eat me” genes CD47 , SIRPA , CD274, PDCD1, LILRB1 and LILRB2 were shown in dot-plot with normalized expression levels of indicated genes from scRNAseq data (n=20) . D . CD47 and PD-L1 were detected on 6 representative PC ascites samples, as examined by CyTOF using antibodies against notable cell type markers, and CD47 and PD-L1. E . CD47 expression (right) on EpCAM + tumor cells detected on PC samples between long-term survivor (>12 month) and short- term survivor (<12 month) (n=42), as assessed by flow cytometry . F . Normalized expression levels of CD47 and SIRPA in tumor cluster and myeloid cluster between long-term survivors and short- term survivors was shown as violin plots G. The overall survival of CD47 in complete responders (CR) GAC patients from TCGA dataset

Galectin-3 synergizes with CD47 to Suppress Phagocytosis and T cell immunity in Peritoneal Metastases of Gastric Adenocarcinoma A B

C

A . Correlation between LGALS3 and CD47 in short-term survivor tumor clusters from scRNA-seq data. B . Gal-3 and CD47 staining on PC samples (n=51) . C . Representative immunohistology staining of Gal-3 and CD47 images in TMA samples (n=210)

Galectin-3 synergizes with CD47 to Suppress Phagocytosis and T cell immunity in Peritoneal Metastases of Gastric Adenocarcinoma

A

C

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A . Representative bioluminescence images over 22 days (left) and quantification (right) of engrafted with KP-Luc2 cell in C57BL/6 mice (n = 5 mice per group) in the indicated treatment. B. Representative contour plots and quantification of phagocytosis at the endpoint day 22, as examined by flow cytometry . C . Schematic illustration of the impact of Gal-3 and CD47 in phagocytosis and immune regulation in GAC in TME

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