Rapid detection of murine interleukin-6 using aptamer functionalised gold nanoparticles Maria Gomes Baptista 1 , Susan Giorgi-Coll 2 , Keri L. H. Carpenter 2 , Jelena Gavrilovic 3 , Simone Dedola 4 , Robert A. Field 4,5 and María J. Marín 1 1 School of Chemistry, University of East Anglia, UK , 2 Department of Clinical Neurosciences, Division of Neurosurgery, University of Cambridge, UK, 3 School of Biological Sciences, University of East Anglia, UK, 4 Iceni Glycoscience Ltd, Norwich Research Park Innovation Centre, UK, 5 Department of Chemistry and Manchester Institute of Biotechnology, UK Interleukin-6 (IL-6) is a cytokine that works as an immunological signalling biomarker in acute inflammation and can be associated with several sepsis-related diseases. 1 Due to their pivotal role in inflammatory biological environments, there is a need for a quick and reliable method to detect this protein. A lateral flow device (LFD, Figure A ) is a diagnostic tool with potential for the rapid detection of biomarkers that can be related to diseases. LFDs take advantage of the colorimetric capacity of gold nanoparticles (AuNPs), using them as carriers of (bio) receptors able to detect the biomarkers of interest ( Figure B ). 2,3 Further to their optical properties, AuNPs present high surface-to-volume ratio, which allows them to be functionalised with various types of ligands increasing their potential for biomedical applications. 4
This work aims to develop a LFD for the quick detection of murine IL-6 using aptamer functionalised AuNPs. For this purpose, AuNPs were synthesised and compared to commercially available ones to evaluate their similarity in sizes and shapes. The synthesised AuNPs were functionalised using different ratios of polyethylene glycol (PEG) and aptamers. The latter consists of ssDNA structures of a complimentary sandwich pair that target different moieties of murine IL-6, which will allow multiple bindings to the protein. The ability of the aptamer functionalised AuNPs to detect murine IL-6 was investigated using two distinct assays: 1) in solution via an aggregation assay and 2) on a nitrocellulose membrane via a dipstick assay. References 1. S. Giorgi-Coll, M. J. Marín, O. Sule, P. J. Hutchinson and K. L. H. Carpenter, Microchimica Acta, 2020, 187 , 1–11. 2. C. Parolo, A. Sena-Torralba, J. F. Bergua, E. Calucho, C. Fuentes-Chust, L. Hu, L. Rivas, R. Álvarez-Diduk, E. P. Nguyen, S. Cinti, D. Quesada-González and A. Merkoçi, Nature Protocols , 2020, 15 , 3788–3816. 3. M. Bauer, M. Strom, D. S. Hammond and S. Shigdar, Molecules, 2019, 24 , 4377. 4. S. Alex and A. Tiwari, Journal of Nanoscience and Nanotechnology, 2015, 15 , 1869–1894. 5. S. Assadollahi, C. Reininger, R. Palkovits, P. Pointl and T. Schalkhammer, Sensors, 2009, 9 , 6084-6100.
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