Performance evaluation of monofunctionalised nonporous silica materials in relation with particle morphology and column dimensions by RP-HPLC Sandhyarani Analakkattillam 1 , Dr. Eric Moore 2 and Dr. John P. Hanrahan 3 1 PhD Student, School of Chemistry, Ireland, Analytical Development Chemist / IRC EBP research scholar, Ireland, 2 Senior Lecturer, School of Chemistry, University College Cork, Ireland, 3 Chief Technical Officer, Glantreo Limited, Ireland, 119222596@ucc.umail, s.analakkattillam@glantreo.com, sandhyaanalakkat@gmail.com, e.moore@ucc.ie, j.hanrahan@glantreo.com A comparative study of the performance characteristics of monofunctionalised nonporous silica materials based on the morphology and the column dimensions were presented in detail. Nonporous silica particles of different particle diameters ranging from 1.3µm to 5µm were studied where the materials were functionalised with mono- functional octadecylmethyldichlorosilane (C 19 H 40 Cl 2 Si) ligand under optimized reflex conditions and packed in- house in different chromatographic columns of dimensions 50 mm L x 2.1 mm ID to 150 mm L x 4.6 mm ID. The synthesized monofunctionalised nonporous particles were characterized by using scanning electron microscopy (SEM) for particle diameter and particle distribution, Brunauer-Emmett-Teller (BET) analysis for surface area, and elemental analysis (CHN) for carbon percentage. The mobile phase and flow rate were optimized using a standard mixture of uracil, toluene, and naphthalene where the reduced plate height of naphthalene was considered for plotting the van Deemter curve. Performance characteristics were evaluated using a mixture of parabens which constitutes methyl, ethyl, isopropyl, propyl, isobutyl, and butyl parabens. Compared the separation and retention efficiencies of each column and the results were discussed in detail. Column performance was evaluated using a High performance liquid chromatographic instrument and with a mobile phase of acetonitrile/0.1% acetic acid in water v/v in different compositions. Detection was made at 254nm and an injection volume of 1µL used. Finally compared the test results with SOLAS™ ODS 100Å 3µm and 5µm and Superficially porous Eiroshell™ 100Å 2.6µm C18 columns of same column dimensions.
IP27
© The Author(s), 2022
Made with FlippingBook Learn more on our blog