Quantitation of amino acids and acylcarnitines in dried blood spots by paper-spray ionisation mass spectrometry (PS-MS) Garcia-Rojas, NS a , Soto-Rodriguez, LD a , Guillen-Alonso a , H, Winkler, R a* , Wishart DS b* a Departamento de Biotecnología y Bioquímica, y Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), México, b Department of Computing Science and Department of Biological Sciences, University of Alberta, Canada In clinical assays, endogenous metabolites are quantified routinely in blood to screen for inborn or acquired metabolic disorders. Changes in amino acid (AA) and acylcarnitine (AC) profiles are characteristic of different diseases, for example, kidney or liver problems, metabolic syndrome, and insulin resistance. However, the logistics of liquid blood samples is complicated and costly because of the requirement of cooling. In contrast, dried blood spots (DBS), taken from a fingerpick, are stable for several months and can be shipped with regular postal services. Extracting the metabolites from DBS for conventional liquid chromatography coupled to mass spectrometry (LC- MS) is tedious and costly. Thus, we evaluated the use of paper-spray ionisation mass spectrometry (PS-MS). The sensitivity of PS-MS methods for endogenous metabolites depends on their desorption from the paper substrate. Thus, we evaluated the factors involved in the desorption and spray formation in PS-MS. As proof-of-concept, we analyse AAs and ACs from DBS samples of male rats with metabolic disorders. For high-throughput analyses, we employ the robotic platform "Open LabBot", which can be easily reproduced by other laboratories, and adapted to different MS instruments. Acknowledgements: The work was supported by the bilateral grant Conacyt-DFG 2016/277850 References 1. Freeman, J. D., Rosman, L. M., Ratcliff, J. D., Strickland, P. T., Graham, D. R., & Silbergeld, E. K. (2018). State of the Science in Dried Blood Spots. Clinical Chemistry , 64 (4), 656–679. https://doi.org/10.1373/clinchem.2017.275966 2. Rosas-Román, I., Ovando-Vázquez, C., Moreno-Pedraza, A., Guillén-Alonso, H., & Winkler, R. (2020). Open LabBot and RmsiGUI: Community development kit for sampling automation and ambient imaging. Microchemical Journal , 152 , 104343. https://doi.org/10.1016/j.microc.2019.104343 3. Moreno-Pedraza, A., Rosas-Román, I., Garcia-Rojas, N. S., Guillén-Alonso, H., Ovando-Vázquez, C., Díaz-Ramírez, D., Cuevas-Contreras, J., Vergara, F., Marsch-Martínez, N., Molina-Torres, J., & Winkler, R. (2019). Elucidating the Distribution of Plant Metabolites from Native Tissues with Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging. Analytical Chemistry , 91 (4), 2734–2743. https://doi.org/10.1021/acs.analchem.8b04406
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