3D PS-MS: 3D printed paper spray ionisation platform coupled to mass spectrometry for automated chemical analyses Leonardo Daniel Soto-Rodríguez 1,2 , Nancy Shyrley García-Rojas 1,2 and Robert Winkler 1,2 1 Center for Research and Advanced Studies (CINVESTAV) Irapuato, Department of Biochemistry and Biotechnology, Mexico, 2 National Laboratory of Genomics for Biodiversity, CINVESTAV IPN, Mexico. Ambient Ionisation Mass Spectrometry (AIMS) allows chemical analyses with minimal technical requirements. Paper Spray Ionisation Mass Spectrometry (PS-MS) recently gained relevance for analysing biological fluids. We designed 3D printed cartridges and adaptors for PS-MS with the robotic platform "OpenLabBot" 1 . We modelled the PS-MS cartridges, high voltage applicator, and multiplex support for measuring ten samples per analytical run. For evaluating the chemical and ambient noise of different polymeric materials, we tested the 3D printing materials polylactic acid (PLA) and acrylonitrile butadiene styrene (ABS). In addition, we experimented with different technical parameters, such as voltage, paper type, paper tip angle, and the distance between the paper tip to the inlet of the mass spectrometer. None of the tested 3D printing polymers generated significant chemical noise. However, the PS-MS cartridges printed with PLA were more solvent-resistant than the ABS versions. We detected caffeine, epicatechin, isoamyl acetate, furan-2-carboxaldehyde, acetaminophen and diclofenac using the PS-MS platform. Currently, we test the PS-MS platform for different applications, such as dried blood spot (DBS) screening and quality control of agave-derived spirits. The 3D PS-MS platform is based on open hardware components and free software and, thus, could be adapted to different uses at a low cost. Acknowledgements: The work was supported by the bilateral grant Conacyt-DFG 2016/277850 References 1. Rosas-Román, I., Ovando-Vázquez, C., Moreno-Pedraza, A., Guillén-Alonso, H. & Winkler, R. Open LabBot and RmsiGUI: Community development kit for sampling automation and ambient imaging. Microchem. J. 152 , 104343 (2020).
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