LC-MS based characterization of post-transcriptional modification profiles under Fenton reaction induced oxidative stress Satenik Valesyan, Balasubrahmanyam Addepalli and Patrick Limbach University of Cincinnati, USA Oxidative stress caused by endogenous and exogenous sources has been associated with a number of human diseases. One of exogenous sources is ultraviolet radiation (UVR). Our lab has previously demonstrated the adverse effects of UVR on tRNA modifications. Fenton reaction is another source of oxidative stress where Fe2+ catalyzes the generation of hydroxyl radicals (HO.) from hydrogen peroxide (H2O2). Our current study documents the effects of cellular exposure to H2O2 with and without additional Fe2+ on post-transcriptional modifications of Escherichia coli (E. coli) RNA through liquid chromatography coupled with mass spectrometry. We notice altered profiles in tRNA modifications that could play important role in influencing the translational accuracy, efficacy, and structural stability. The implications of these findings in contrast with the UVR exposure effects will be discussed. References 1. Liguori et al. (2018) Clin Interv Aging. 13: 757–772. 2. Pizzino G. et al. (2017) Oxid Med Cell Longev. 2017: 8416763. 3. Sun et al. (2018) ACS Chem Biol., 13(3): 567. 4. Latunde-Dada G. et al. (2019) Int. J. Mol. Sci.,20(19), 4968. 5. Dedon et al. (2010) PLoS Genet., 6(12): e1001247.
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