A turn-on fluorescent probe for detection of In3+ based on benzylidene pyridyl semicarbazide derivative: application in live imaging in zebrafish Mujthaba Aatif A and S.K. Ashok Kumar* 1 Vellore Institute of Technology, India A newly designed benzylidene pyridyl semicarbazide ( L ) probe was synthesized by reacting pyridine-2,6- dicarbohydrazide with 2-hydroxy-3-methylbenzaldehyde and structurally characterized by using NMR, FTIR, and HR-MS analysis. The sensing ability of probe L was studied with various metal ions by using visual, UV Visible, and spectrofluorometric methods. It shows a turn-on emission fluorescent at 492 nm for In 3+ over other tested ions. The probe L exhibits a 2:1 stoichiometry complex formation with In 3+ with an estimated association constant (K a ) of 1.417×10 3 M -1 determined by the BindFit method. The limit of detection (LOD) of In 3+ using L was found to be 83.2 nM. The binding mechanism of L with In 3+ was characterized by using 1 H NMR, ESI mass, and DFT analysis. The test paper strip experiments were conducted to demonstrate the practical utility of the proposed probe. Furthermore, probe L was applied for the analysis of In 3+ content present in various real water samples and successfully used to trace In 3+ in living cells and zebrafish. References 1. Asami, A. Yoshino, M. Kubota and S. Gotoh, Zeitschrift für Pflanzenernährung und Bodenkunde , 1990, 153 , 257–259. 2. J. Jang, J. H. Kang, D. Yun, and C. Kim, Photochemical & Photobiological Sciences , 2018, 17 , 1247–1255. 3. A. Majeed, K. S. N. Nambi, G. Taju, S. Vimal, C. Venkatesan, and A. S. S. Hameed, Environmental Science and Pollution Research , 2014, 21 , 13539–13550. 4. H. Wang, D. R. Gao, X. Y. Wang, Y. M. Lu, W. X. Shen, and Y. Y. Lv, Sensors and Actuators, B: Chemical , 2019, 294 , 14–23. 5. Erbas-Cakmak, S. Kolemen, A. C. Sedgwick, T. Gunnlaugsson, T. D. James, J. Yoon and E. U. Akkaya, Chemical Society Reviews , 2018, 47 , 2228–2248.
V23
© The Author(s), 2022
Made with FlippingBook Learn more on our blog