Confirmation of Juvenile Hormone Binding Protein Gene Sequences and Expression in Codling Moth ( Cydia pomonella )
Ruby Zepeda, Jasmin Gonzalez, Sephen Martinez Project Mentor(s): Aram Langhans, William Walker
The codling moth ( Cydia pomonella ) is a common pest in agriculture that targets apple and pear orchards, and its larvae pose a threat to the growth and production of fruit in Eastern Washington and around the world. This research aims to identify and confirm key genes responsible for the production of Juvenile Hormone Binding Protein (JHBP), an essential protein for the moths’ development. With confirmed sequences from these studies, future research can build on ways to disrupt JHBP expression so it might stop the growth of the larvae before it eats its way into the fruit. Using PCR, we copied the cDNA, cloned, and sequenced the genes to determine if the full-length sequences match the ones that are predicted in the codling moth DNA database. At this point in the project, sequence analysis indicated that nine out of eleven of our sequences aligned with those predicted in the codling moth database. Presentation Type: Poster Presentation (May 21, 9:30am–3:00pm) Keywords : Codling Moth, Gene Sequencing, Juvenile Hormone Binding Protein, Larval Development SOURCE Form ID: 8N
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