PRE CLINICAL SCIENCE
EXPOSURE TO LOW FREQUENCY CMF ™ RESULTED IN A SIGNIFICANT INCREASE IN CELLULAR CA+ FLUX FITZSIMMONS RJ, RYABY JT, MAGEE FP, BAYLINK DJ
SUMMARY: The in vitro study investigated the lower amplitude CMF ™ effect on osteoblast-like bone cells (TE-85 and SaOS-2), a common model used to measure electrical stimulation cellular effect. The impact of the CMF signal was determined by measuring Ca uptake into the bone cells, or Ca flux. The authors also examined the differentiation state of the bone cells by examining the alkaline phosphatase (ALP) levels. Both a time and frequency dependent response was noted by the cells to the signal. The Ca uptake increased with increased exposure to the signal for up to 40 minutes. The optimal frequency for uptake was observed to be between 15.3Hz and 16.3Hz. Also, these trends were not dependent on the state of differentiation of the bone cells.
“ SIGNIFICANT INCREASE IN CELLULAR CA+ FLUX ”
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