RSC Tokyo International Conference 2023
As the representative works, the author developed a printed 384-well microtiter PCSAD to simultaneously categorize and discriminate food-related analytes (i.e., saccharides and sulfur-containing amino acids) that play roles as not only essential taste components but also markers to indicate food freshness. 4 The optimized 384-well microtiter PCSAD requiring 1 μL/4 mm 2 of each well can be manufactured using a common office printer, which could accelerate the establishment of easy-to-fabricate chemical sensor devices in real-world scenarios (Figure 2(a)). In addition, off-the-shelf materials were employed as building blocks for fluorescent chemosensors to avoid the synthetic burden. The self-assembled fluorescent chemosensors embedded with the 384-well microtiter PCSAD showed various fluorescence response patterns upon adding analytes, which indicated the cross-reactivity of the self- assembled chemosensors (Figure 2(b)). The paper-based chemosensor array system combined with imaging analysis and pattern recognition techniques not only successfully categorized saccharides and sulfur-containing amino acids but also classified mono- and disaccharide groups (Figure 2(c)). Furthermore, the 384-well microtiter PCSAD performed a regression analysis of fructose (Fru) and glutathione (GSH) in diluted freshly made tomato juice (Figure 2(d)), which revealed high quantitative detectability. Judging from the versatility of the solid- state optical chemosensor array devices, the author believes that the proposed sensor designs for real-sample analysis could maximize the potential of self-assembled optical materials.
Figure 2. (a) Photograph of the manufactured 384-well microtiter PCSAD and schematic illustration of a capturing system for fluorescent images. (b) A digitally expanded fluorescent image of the PCSAD by adding the analytes. (c) Result of the qualitative analysis against 13 analytes. (d) Results of the quantitative analysis against Fru and GSH in a diluted freshly made tomato juice.
References 1. T. Minami et al., “Molecular self - assembled chemosensors and their arrays”, Coord. Chem. Rev., 429 , 213607 (2021). 2. T. Minami et al., “Supramolecular optical sensor arrays for on - site analytical devices”, J. Photochem. Photobiol. C 51 , 100475 (2022). 3. T. Minami et al., “96 -well Microtiter Plate Made of Paper: A Printed Chemosensor Array for Quantitative Detection of Sac charides”, Anal. Chem . 93 , 1179 (2021). 4. T. Minami et al., “Printed 384 -Well Microtiter Plate on Paper for Fluorescent Chemosensor Array in Food Analysis”, Chem. Asian J . 17 , e202200597 (2022).
RSC Tokyo International Conference, Makuhari Messe, Chiba, Japan, September 7-8, 2023.
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