RSC Tokyo International Conference 2023
Material based Induction of Neural Stem Cell Spheroid and the Application of Brain on a Chip
I-Chi Lee Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 300044, Taiwan
Biographical Sketch
Professor Lee currently holds the position of Associate professor at the Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University. Dr. I Chi Lee received her Ph.D. from the Department of Biomedical Engineering, National Taiwan University in 2007. She then worked as a postdoctoral research fellow at the genomics research center, Academia Sinica in Taiwan, and, subsequently, served as Assistant Professor, Associate Professor, and Professor at the Graduate Institute of Biomedical Engineering, Chang Gung University. Her research focuses on biomaterials, tissue engineering, drug delivery, and organ on chip. She has published over 50 peer-reviewed articles and one book (ISBN: 978-1-63485-878-6) and has received several important research awards in Taiwan, including Ta-You Wu Memorial award in 2018. Recently, her efforts have been directed towards translating knowledge on fabricating organ-on-chip and in vitro models using 3D bioprinting. The development of these in vitro models holds great potential for advancing precision medicine, personalized medicine, and reducing reliance on animal experimentation.
Abstract
The regulation of neural
stem/progenitor
cell the
(NSPC) guidance
niches,
of
neurite
outgrowth, the establishment of neural networks, along with the induction of functional neurons, are pivotal components in the progression of neural and
engineering. Moreover, stem cell-based in vitro models hold potential for therapeutic strategies and drug screening for neurodegenerative disorders, such as Alzheimer's disease (AD). In this study, a novel approach was employed to fabricate a niche-modulated system using polypeptide multilayer films, enabling investigation into the effects of surface properties on NSPC differentiation. Additionally, a biomimetic system consisting of supported lipid bilayers (SLBs) with adsorbed sequential multilayer films was developed to closely mimic the natural environment, resembling synaptic membranes. Furthermore, a biochip based on NSPC spheroids, which exhibited a 3D brain-like structure, well-defined neural differentiation, and neural network formation, was created. This brain-on-a-chip model, specifically tailored towards AD research, was constructed using microelectrode arrays with a multichannel platform, allowing real-time monitoring of neural network formation and degeneration through impedance analysis. The AD-on-a-chip model offers enhanced insights into neurological pathology, and its development provides an alternative approach for studying drug discovery and cell-protein interactions within the brain.
RSC Tokyo International Conference, Makuhari Messe, Chiba, Japan, September 7-8, 2023.
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