RSC Tokyo International Conference 2023
Development of Microfluidic Biosensor with Smartphones and its Application in Rapid Detection of Foodborne Pathogens Ling LIN, Gaowa XING, Yuting SHANG, Xiaorui WANG Department of Bioengineering, Beijing Technology and Business University, CHINA
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Biographical Sketch
Professor Ling Lin received her Ph.D. degree at The University of Tokyo in 2016, working on nano/microfluidics for living single-cell analysis. She joined the National Center for Nanoscience and Technology as an assistant professor in 2016 and got promoted to associate professor in 2020. Since 2021 she has been a professor at the Department of Bioengineering, Beijing Technology and Business University, and She was selected for Beijing Nova Program, in China in 2022. Her current research interest is the development of liquid crystal biosensors for the detection of ultra-micro components in the process of living single cells, integrated microfluidic chips for the analysis of cell drug metabolism, and rapid detection of pathogenic bacteria.
Abstract
Foodborne pathogens have raised significant concerns in human public health. Every year, millions of people in the world are infected by food and water contaminated by pathogenic bacteria, resulting in various diseases that endanger human health. The traditional culture- based detection method usually takes 2-3 days or more. The detection methods based on molecular biology and immunology reduce the detection time compared with the traditional method but still have problems such as complex operation, dependence on professional laboratories and technicians, high detection cost, and low detection flux. Therefore, rapid, high- sensitive, low-cost, and easy-to-use testing methods for food safety are needed. Microfluidic technology with flexible operation, high throughput detection, low reagent consumption, and easy integration with miniaturized instruments has been applied in many fields [1-3] . Based on the principles of immunology and nucleic acid amplification and detection, this dissertation aims to develop microfluidic biosensors for rapid, high-throughput, and highly sensitive detection of foodborne pathogens. The microfluidic biosensors integrated microfluidic, optical, magnetic separation, nano-material fluorescence quenching, and nano-enzyme catalytic amplification technologies, and combined with smartphones. This reports mainly includes the following innovative works: Multiplexed detection of foodborne pathogens using one-pot CRISPR/Cas12a combined with recombinase-aided amplification on a finger-actuated microfluidic biosensor (Figure 1) [4] . The finger-actuated microfluidic chip and a smartphone were used to develop a finger- actuated microfluidic biosensor to realize a one-pot RAA-CRISPR/Cas12a assay for seven common foodborne pathogens detection, including Escherichia coli O157: H7 Staphylococcus aureus , Bacillus cereus , Pseudomonas aeruginosa , Cronobacter sakazakii , Listeria monocytogenes , Vibrio parahaemolyticus and Salmonella .
RSC Tokyo International Conference, Makuhari Messe, Chiba, Japan, September 7-8, 2023.
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