RSC Tokyo International Conference 2023

RSC Tokyo International Conference 2023

Figure 1. Illustration of the multiplexed detection of foodborne pathogens based on one-pot RAA- CRISPR/Cas12a assay on finger-actuated microfluidic biosensor (FA-MB)

Microfluidic biosensor based on enzyme-modified MOF as multiple-enzyme mimetic nanoreactors for sensitive detection of Escherichia coli O157: H7 (Figure 2). Pt-PCN-224 as a nano enzyme material with three mimic enzyme activities of oxidase, peroxidase, and catalase was easily to be covalently modified with antibodies of target bacteria. The triple mimic enzyme activities were applied for synergetic catalytic signal amplification and combined with a smartphone to develop a microfluidic biosensor for Escherichia coli O157: H7 detection.

Figure 2. Illustration of microfluidic biosensing of foodborne bacteria based on Pt-PCN-224 with POD catalytic signal amplification.

References

1. Q. Zhang, S. Feng, L. Lin*, S. Mao, J-M. Lin*, Emerging open microfluidics for cell manipulation, Chem. Soc. Rev., 50, 5333- 5348 (2021). 2. W. Li, M. Khan, L. Lin*, Q. Zhang, S. Feng, Z. Wu, J-M. Lin*, Monitoring H2O2 on the Surface of Single Cells with Liquid Crystal, Angew. Chem. Int. Ed., 59, 9282-9287 (2020) 3. Z. Wu, Y. Zheng, L. Lin*, S. Feng, Z. Li, J-M. Lin*, Controllable Synthesis of Multicompartmental Particles Using 3D Microfluidics, Angew. Chem. Int. Ed., 59, 2225-2229 (2020) 4. G. Xing, Y. Shang, X. Wang, H. Lin, S. Chen, Q. Pu, L. Lin*, Multiplexed detection of foodborne pathogens using one-pot CRISPR/Cas12a combined with recombinase aided amplification on a finger-actuated microfluidic biosensor, Biosens Bioelectron, 220, 114885 (2023).

RSC Tokyo International Conference, Makuhari Messe, Chiba, Japan, September 7-8, 2023.

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