Zero Hunger (SDG 2), Good Health & Well-being (SDG 3)
Molecular networking and targeted isolation of naphthalenoid pigments from the ectomycorrhizal fungus Pisolithus arhizus Armelle Tontsa Tsamo 1* , Pierre Mkounga 1 , Augustin Nkengfack 1 , Mehdi Beniddir 2 1 Department of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box: 812, Yaounde, Cameroon, 2 Équipe “Chimie des Substances Naturelles” BioCIS, CNRS, Université Paris-Saclay, 17 Avenue des Sciences, 91400 Orsay, France E-mail: armelletsamo@yahoo.fr/ armelle.tsamo@facsciences-uy1.cm Pisolithus arhizus is a globally distributed ectomycorrhizal fungus known for its traditional medicine use for wound healing and antimicrobial properties 1 . It is also valued for its role in natural dye production 2 , ecological significance and ectomycorrhizal associations that detoxify polluted soils and support plant growth 3 . In this study, an integrated liquid chromatography-tandem mass spectrometry (LC- MS2)-guided molecular networking strategy was employed to investigate the phytochemical profile of the dichloromethane-methanol crude extract obtained from the fruiting bodies of Pisolithus arhizus (Sclerodermataceae), an ectomycorrhizal fungus. The molecular networking analysis revealed a distinct cluster of naphthalenoid pigments, with several nodes sharing similar parent mass. Annotation based on LC-HRMS/MS enabled the rapid isolation, structural elucidation and identification of a new naphthalenoid pulvinic acid derivative alongside the previously reported norbadione A (2). Structural characterisation of the compounds was achieved through LC-MS analysis and comprehensive 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. Additionally, the molecular networking analysis uncovered nodes corresponding to unannotated ions, suggesting the presence of potentially novel compounds yet to be characterized. Inspired by previous studies, the in vitro antibacterial activities of the isolated compounds were evaluated against Mycobacterium smegmatis , M. abscessus , M. bovis BCG , Staphylococcus aureus , and Pseudomonas aeruginosa . However, no notable antibacterial activity was observed against the tested bacteria. Key words: Pisolithus arhizus, metabolomics, phytochemical investigation, naphthalenoid Pigments
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References 1. Oliveira, R. S., Preto, M., Santos, G., Silva, A. M., Vasconcelos, V., & Martins, R. (2024). Exploring the bioactive potential of Pisolithus (Basidiomycota): Comprehensive insights into antimicrobial, anticancer, and antioxidant properties for innovative applications. Microorganisms, 12(3), 450. 2. Divya, K., Soosairaj, A. A., Arunmozhi, A. A., & Rajesh, A. L. (2024). Pisolithus arhizus dye: A novel natural dopant for tailoring nonlinear optical properties of guanidinium carbonate single crystals. Chemical Physics Impact, 8, 100591. 3. Ediriweera, A. N., Karunarathna, S. C., Yapa, P. N., Schaefer, D. A., Ranasinghe, A. K., Suwannarach, N., & Xu, J. (2022). Ectomycorrhizal mushrooms as a natural bio-indicator for assessment of heavy metal pollution. Agronomy, 12(5), 1041.
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