Zero Hunger (SDG 2), Good Health & Well-being (SDG 3)
Characterization of compounds isolated from Helichrysum Odoratissimum plant species and their activities on selected oral microbes Madina Mohamed Adia 1* , Osbert Ambassador 1 , Muhammad Azeem 2 , Beatrice Achan 3 , John Baptist Kisitu 3 , Aqsa Rasheed 2 , Peter Ssekandi 1 , Francis Ocheng 4 . 1 Department of Chemistry, Makerere University, P.O Box 7062, Kampala, Uganda. 2 Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan. 3 Department of Medical Microbiology, College of Health Sciences, Makerere University. 4 Department of Dentistry, College of Health Sciences, Makerere University, Uganda. E-mail: madrine1@gmail.com Helichrysum odoratissimum is used for traditional oral health care and its crude extracts demonstrated activity against bacteria that cause Periodontal diseases and dental caries. The essential oil was extracted by hydro-distillation of fresh areal parts and its chemical composition analyzed using Gas chromatography-mass spectrometry (GC-MS). Crude extracts were obtained by maceration extraction of dried plant material. Chromatographic and spectroscopic techniques were used in isolation and characterization of compounds. Antibacterial and antifungal activities were investigated through determination of minimum inhibitory concentration and minimum bactericidal concentration. The essential oil was dominated by sesquiterpene hydrocarbons like α-caryophyllene (17.5%), trans-β- caryophyllene (16.8%) and monoterpenes like α-pinene (15.8%). Two flavones: 3-O-methylquercetin (1) and 3,5-dihydroxy-6,7,8-trimethoxyflavone (2) and two sterols: β-sitosterol (3) and stigmasterol (4) were isolated from H. odoratissimum and were found active on Streptococcus mutans , Streptococcus aureus and Candida albicans . It is the first time compounds 3 and 4 are isolated from the plant H. odoratissimum . The extracts of H. odoratissimum contain phytochemicals that are active on oral microbials which justifies its use as a traditional medicine for oral health care. The study is in line with SDG no. 3 which is good health and wellbeing of community members who use the plant in oral care. Key words: Helichrysum odoratissimum; Dental caries; Periodontal diseases; Oral microbials; Essential oil.
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