Zero Hunger (SDG 2), Good Health & Well-being (SDG 3)
Phytochemical investigations and antioxidant properties of methanolic extracts from Senecio asperulus and Cussonia paniculata
Selloane Masiloane, * Mohale Mabaleha National University of Lesotho. Email: castalinamasiloane@gmail.com
C. paniculata leaves, roots and aerial parts of S. asperulus were macerated with methanol respectively. The phytochemical screening of the crude methanol extract of C. paniculata showed many class compounds including flavonoids and phenolic compounds while S. asperulus aerial parts had alkaloids, tannins and terpenoids and S. asperulus roots revealed tannins, alkaloids and anthraquinones. The MeOH crude extract of C. paniculata showed moderate radical scavenging activity percentage averaging 51.4 µg/ml following the positive control, ascorbic acid at 90.5 µg/ml. S. asperulus aerial parts’ activity averaged 25.5 µg/ml at the highest concentration. MeOH crude extracts went through sequential fractionation in open column chromatography with hexane, dichloromethane, ethyl acetate (EtOAc), acetone and 10% aqueous methanol. Three compounds 1, 2 and 3 were isolated from respective EtOAc fractions and purified using Thin Layer Chromatography and Column Chromatography. 1D and 2D NMR techniques were employed for structural elucidation. Compound 1 (9.9 mg), isolated from the EtOAc fraction of C. paniculata leaves showed characteristics of C 30 carbon triterpenoid was identified as a known pentacyclic triterpenoid uvaol. Compound 2 (68.5 mg), isolated from EtOAc fraction of the S. asperulus roots and compound 3 (320.3 mg) from S. asperulus aerial parts as sesquiterpenoids type 8,12-epoxy-9-hydroxy-6[(senecioyl)oxy] eremophil-7,11-dien-1-one and 8,12-epoxy-9-hydroxy-6[(butyryl)oxy]eremophil-7,11-dien-1-one, respectively. Key words: S. asperulus, C. paniculata, chromatography, NMR
P21
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