DISCRETE ANALYZER CHEMISTRY Nitrate/Nitrite (Hydrazine Reduction) Method
Methods Referenced • SEAL Analytical AQ400 Method EPA-141-C Rev. 1A
• EPA Method 353.1 Rev 2.0 • Standard Methods 4500-NO 3 - H
Reagent Composition Three Reagents are used for the Nitrate/Nitrite (Hydrazine Reduction) method: Reagent 1: SEALAnalyticalAQ NOx Hyd-Buffer – Alkaline Buffer Reagent in DI Water: 46 g/L Sodium Hydroxide 25 g/L Sodium phosphate dibasic Reagent 2: SEALAnalyticalAQ NOx Hydrazine – Working Hydrazine Reagent in DI Water: 1.875 g/L Hydrazine Sulfate 0.0125 g/L Copper (II) Sulfate pentahydrate 0.2 g/L Zinc (II) Sulfate heptahydrate Reagent 3: SEALAnalyticalAQ NOx Hyd-Color – Sulfanilamide-NEDD Reagent in DI Water 4% v/v Phosphoric Acid 6 g/L Sulfanilamide 0.3 g/L N-(1-naphthyl)-ethylenediamine dihydrochloride We recommend ICNNO31 and ICNNO21 to make your standards and reduction efficiency check solutions for this method. These solutions are already certified as Nitrate or Nitrite as Nitrogen, so no conversions are needed, just simple dilution factors to get these diluted to your working concentrations. This method covers the determination of Nitrate-N and Nitrite-N in sewage and effluents, raw and finished drinking waters, and industrial wastes. Method Range 0.02 – 1.5 mg N/L
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