Electrospun nanofibers based on marigold extract as promising strategy for wound healing Ovinuchi Ejiohuo 1 , Magdalena Paczkowska-Walendowska 1 , Daria Szymanowska 1,2 , Piotr Kubiak 2 , Andrzej Miklaszewski 3 , Judyta Cielecka-Piontek 1 1 Department of Pharmacognosy, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland, 2 Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 48 Wojska Polskiego Street, 60-627 Poznan, Poland, 3 Institute of Materials Science and Engineering, Faculty of Materials Engineering and Technical Physics, Poznan University of Technology, Jana Pawła II 24, 61-138 Poznan, Poland Calendula officinalis (marigold) shows anti-inflammatory, antibacterial as well as angiogenic, and fibroplastic properties and is beneficial in reducing inflammation and promoting wound healing. Despite the many health- promoting properties of the plant material, the active compounds have low solubility, which may limit their use. One innovative method of improving active compounds' solubility is the production of electrospun nanofibers. Therefore, the work aimed to develop electrospun nanofibers containing lyophilized marigold extract to enhance the solubility of active compounds. Dry hydroalcoholic extract was lyophilized to obtain a powder. Calendulae flos lyophilized extract was standardized with phenolic acids (chlorogenic acids) and flavonoids (narcissin) content. Antioxidant activity was demonstrated by the DPPH and CUPRAC method, anti-inflammatory activity was shown as the degree of inhibition of the hyaluronidase enzyme activity. The structure of the obtained nanofibers was confirmed using a scanning electron microscope (SEM). Physicochemical properties, such as the release profile of the active compounds from the matrix, FTIR, XRPD, and biological properties, such as antioxidant, anti-inflammatory, and microbiological activity of the nanofibers, were also examined was determined as the inhibition of the growth of selected bacteria (including Staphylococcus aureus, Escherichia coli, and Candida albicans ). The PVP/HPBCD and PVP/PCL-basedCalendula encapsulated electrospun nanofibers showed improved physicochemical properties and illustrated the most antioxidant, anti-inflammatory and antibacterial efficiencies. The results obtained through the experimentation of incorporating Calendulae flos lyophilized extract into electrospun nanofibers indicate a high potential for this combination for promoting wound healing. This is due to the combination of the biological properties of Calendulae flos extract and the physical characteristics of the nanofiber, creating a synergistic effect that enhances the wound-healing process. References
1. Chanaj-Kaczmarek J, Paczkowska M, Osmałek T, Kaproń B, Plech T, Szymanowska D, 2. Karaźniewicz-Łada M, Kobus-Cisowska J, Cielecka-Piontek J (2020) Hydrogel Delivery 3. System Containing Calendulae flos Lyophilized Extract with Chitosan as a Supporting 4. Strategy for Wound Healing Applications. Pharmaceutics 12(7): 634.
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