Biofunctionalized ZnS quantum dot/epsilon-poly-L-lysine nanoconjugates for bioimaging brain cancer cells Herman Mansur, Alexandra A. P. Mansur, Sandhra C. Carvalho Center of Nanoscience, Nanotechnology, and Innovation – CeNano2I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, UFMG, Brazil *hmansur@demet.ufmg.br Glioblastoma (GBM) remains the most lethal form of brain cancer, where nanomaterials biofunctionalized with polypeptides offer disruptive strategies relying on passive/active targeting for diagnosis and therapy for fighting cancer cells [1,2]. Thus, in this research, we report for the first time the design and synthesis of novel nanoconjugates composed of photoluminescent ZnS quantum dots (ZnS-QDs) surface functionalized by epsilon- poly-L-lysine (εPL) (ZnS-QD@εPL) produced through a biocompatible and eco-friendly aqueous process for bioimaging and potential antitumor activity in GBM cancer. These nanoconjugates were characterized by their physicochemical and optical properties associated with spectroscopic and morphological features. The results demonstrated that chemically stable photoluminescent ZnS-QD@εPL colloidal nanoconjugates were effectively formed with positively surface-charged features due to the cationic nature of the amine-rich polypeptide. They presented cytocompatibility assessed by MTT assay. Moreover, it is suggested that PLL has promoted cell penetration due to its polycationic nature, demonstrating photoluminescent activity as nanoprobes for bioimaging GBM cancer cells (U87). Thus, it can be envisioned that these novel nanoconjugates offer innovative nanoplatforms for diagnosis and as an adjuvant for GBM anticancer therapy. References 1. Nehra, M.; Uthappa, U.T.; Kumar, V.; Kumar, R.; Dixit, C.; Dilbaghi, N.; Mishra, Y.K.; Kumar, S.; Kaushik, A. Nanobiotechnology-assisted therapies to manage brain cancer in personalized manner. J. Controlled Release 2021, 338, 224-243. https://doi.org/10.1016/j.jconrel.2021.08.027 2. Carvalho, I.C.; Mansur, H.S.; Mansur, A.A.P.; Carvalho, S.M.; de Oliveira, L.C.A.; Leite, M.F. Luminescent switch of polysaccharide-peptide-quantum dot nanostructures for targeted-intracellular imaging of glioblastoma cells. J. Mol. Liq. 2020, 304, 112759. https://doi.org/10.1016/j.molliq.2020.112759 3. Mansur AAP, Carvalho SM, Oliveira LCA, Souza-Fagundes EM, Lobato ZIP, Leite MF, Mansur HS. Bioengineered Carboxymethylcellulose-Peptide Hybrid Nanozyme Cascade for Targeted Intracellular Biocatalytic-Magnetothermal Therapy of Brain Cancer Cells. Pharmaceutics. 2022 Oct 18;14(10):2223. DOI: 10.3390/pharmaceutics14102223.
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