SBMS Newsletter Issue 04

School Highlights

New Equipment

We continuously purchase and install new equipment to facilitate our research and innovation, and we’re happy to introduce the following new members of our equipment family:

Hybridization Oven (ACD/HybEZII System)

Gene Pulser(Biorad/ GenePulserXcellTotal System) Electroporation system for transfection of mammalian cells, bacteria & fungi Location: L4-06, Lab Block Tech-in-charge: Mr. Leonard Cheung leonardc@hku.hk | +852-3917-6832

Gasket-sealed, temperature-controlled benchtop hybridization oven for 20 slides

Location: L4-01, Lab Block Tech-in-charge: Ms. Kary Xu karyxu@hku.hk | +852-3917-9268

Sonifier(Emerson/SFX550) Ultrasonic homogenizer system for liquid processing from 0.2 to 1000ml

Location: L4-03, Lab Block Tech-in-charge: Ms. Sandra Wong syywong@hku.hk | +852-3917-6926

Publication Highlights *Co-corresponding Authors

Guo W, Kinghorn AB , Zhang Y, Li Q, Poonam AD, Tanner JA *, Shum HC*. Non-associative phase separation in an evaporating droplet as a model for prebiotic compartmentalization. Nat Commun. 2021 May 27;12(1):3194. Zhang T, Pramanik G, Zhang K, Gulka M, Wang L, Jing J, Xu F, Li Z, Wei Q, Cigler P*, Chu Z *. Toward Quantitative Bio-sensing with Nitrogen-VacancyCenterin Diamond. ACS Sens. 2021 May 26. doi:10.1021/acssensors.1c00415. Yu J, Oentaryo MJ, Lee CW . Local protein synthesis of neuronal MT1-MMPforagrin-induced presynaptic development. Development. 2021 May15;148(10):dev199000. Feng D, Wang J, Yang W , Li J, Lin X, Zha F, Wang X, Ma L, Choi NT , Mii Y, Takada S, Huen MS , Guo Y, Zhang L, Gao B . Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation. Sci Adv. 2021 May14;7(20):eabg2099. Feng Z, Yu CH . PI(3,4)P2-mediated membrane tubulation promotes integrin trafficking and invasive cell migration. Proc Natl Acad Sci USA. 2021 May11;118(19):e2017645118. Huang X, Huang Y . Cellsnp-lite: an efficient tool for genotyping single cells. Bioinformatics. 2021 May 8:btab358. Lin Q, Rong L , Jia X, Li R, Yu B , Hu J, Luo X, Badea SR, Xu C , Fu G, Lai K, Lee MC , Zhang B, Gong H , Zhou N, Chen XL, Lin SH*, Fu G*, Huang JD *. IFN- γ -dependent NK cell activation is essential to metastasis suppression by engineered Salmonella. Nat Commun. 2021 May 5;12(1):2537. Tan SZ, Poon CH, Chan YS, Lim LW . Prelimbic Cortical Stimulation Disrupts Fear Memory Consolidation through Ventral Hippocampal Dopamine 2 Receptors. Br J Pharmacol. 2021 Apr 26. doi: 10.1111/bph.15505. Loong JH, Wong TL, Tong M , Sharma R, Zhou L, Ng KY, Yu HJ, Li CH , Man K, Lo CM, Guan XY, Lee TK, Yun JP, Ma S . Glucose deprivation induced aberrant FUT1-mediated fucosylation drives cancer stemness in hepatocellular carcinoma. J Clin Invest. 2021 Apr 20:143377. Chan A, Ma S , Pearson BJ, Chan D . Collagen IV differentially regulates planarian stem cell potency and lineage progression. Proc Natl Acad Sci U S A. 2021 Apr 20;118(16):e2021251118. Zhao L, Gao X , Zheng Y, Wang Z, Zhao G, Ren J, Zhang J, Wu J , Wu B, Chen Y, Sun W, Li Y, Su J, Ding Y, Gao Y, Liu M, Bai X, Sun L, Cao G, Tang F, Bao S, Liu P *, Li X*. Establishment of bovine expanded potential stem cells. Proc Natl Acad Sci USA. 2021 Apr 13;118(15):e2018505118. Buang N, Tapeng L, Gray V , Sardini A, Whilding C, Lightstone L, Cairns TD, Pickering MC, Behmoaras J, Ling GS *, Botto M*. Type I interferons affect the metabolic fitness of CD8+ T cells from patients with systemic lupus erythematosus. Nat Commun. 2021 Mar 31;12(1):1980.

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