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Summary: Clemson University Field and Greenhouse studies evaluated the effect of several products (Table 1) on rooting characteristics, thatch depth, and thatch weight of ‘Diamond’ zoysiagrass grown in an 85:15 sand:peat mix meeting USGA greens mix specifications. In the greenhouse study, no differences in root weight were detected in year one. In year two, EarthMAX applied at 1 gallon/acre on a 14-day interval increased root weight compared to all treatments (Figure 1). In the field study, EarthMAX applied at 1 gallon/acre on a 14-day interval increased root length ≥28% over blackstrap molasses and the untreated control in both years (Figure 2). In year two, the same treatment increased root mass ≥97% versus the control (Figure 3). Blackstrap molasses increased root weight compared to the untreated control >500% in both years (Figure 3). In the field study, treatments did not have an effect on thatch weight compared to the nontreated control. Thatch thickness in the nontreated control was ≥42% than EarthMAX applied at 2 gallon/acre and blackstrap molasses applied weekly. Thatch thickness in treatments receiving sand topdressing was ≥48% than all other treated plots across both years (Figure 4). Evaluation of Biological Thatch Control on Golf Greens Research Summary Summary: Clemson University Field and Greenhouse studies evaluated the effect of several products (Table 1) on rooting characteristics, thatch depth, and thatch weight of ‘Diamond’ zoysiagrass grown in an 85:15 sand:peat mix meeting USGA greens mix specifications. In the gr enhouse stu y, no differences in root w ig t were detect d in year one. In year tw , Eart MAX applied at 1 gallon/acre on a 14-day interval increased root weight compared to all treatments ( Figure 1 ). In the field study, EarthMAX applied at 1 gallon/acr on a 14-day interval increased root length ≥ 28% over blackstrap molasses and the untreated control in both years ( Figure 2 ). In year two, the same treatment increased root mass ≥ 97% versus the control ( Figure 3 ). Blackstrap molasses increased root weight compared to the untreated control >500% in both years ( Figure 3 ). In the field study, treatments did not have an effect on thatc weight compar d to the nontreated control. Thatch thick ess in the nontreated control was ≥ 42% than EarthMAX applied at 2 gallon/acre and blackstrap molasses applied weekly. Thatch thickness in treatments receiving sand topdressing was ≥ 48% than all other treated plots across both years ( Figure 4 ). EVALUATION OF BIOLOGICAL THATCH CONTROL ON GOLF GREENS RESEARCH SUMMARY eral p u ts (T l 1) on rooting characteristics, thatch depth, and thatch weight of ‘Diamond’ zoysiagrass grown in an 85:15 sand:peat mix m i USGA greens mix i ic i s. I t r dy, i in ei er e i . I w , p lied gallon/acre on a 14-day interval increased root weight compared to all treatments ( ig . In the field st , EarthMAX applied all / n in l i e e o l % er l l d ol i r igu . I a t , h e i s 7% h l i e . la p l s i d ig ed t l i h ( i r . I i ld s y, t di n e n i e c ol. h t t i i n t d n rol n r li ll l l li l . i i i i i ll l i . l . n e i l s l i i ' i ' i i , i , weight. T a A li i U - Wo 6 fl 1 3 l i i i l i ev luated e e

Table 1. Treatments for a greenhouse and field study evaluating the effects of various products on 'Diamond' zoysiagrass rooting, thatch thickness, and thatch weight. Treatment Rate Application Frequency Untreated -- -- Worm Power 16 fl oz/1000 sq ft 30 days EarthMAX (1) 1 gal/acre 14 days EarthMAX (2) 2 gal/acre 30 days Blackstrap Molasses 5.25 fl oz/1000 sq ft 7 days Sand Topdressing* 0.6 mm 14 days *Sand topdressing was applied by hand Treatment Rate Application Frequency Untreated -- -- Worm Power 16 fl oz/1000 sq ft 30 days EarthMAX (1) 1 gal/acre 14 days EarthMAX (2) 2 gal/acre 30 days Blackstrap Molasses 5.25 fl oz/1000 sq ft 7 days Sand Topdressing* 0.6 mm 14 days *Sand topdressing was applied by hand Table 1. Treatments for a greenhouse and field study evaluating the effects of various products on ‘Diamond’ zoysiagrass rooting, thatch thickness, and thatch weight. l l Bl l . l 7 Sand To i . *Sand topdressing was applied by hand

Figure 3. Turfgrass root weight for two 16-week thatch control field studies. Means fo significantly different according to Fisher's protected LSD (a=0.10). The Walker Golf Co Figure 3. Turfgrass root weight for two 16-week thatch control field studies. Means followed by the same letter within each year are not significantly different according to Fisher’s protected LSD (a=0.10). The Walker Golf Course, Clemson, SC. Figure 3. Turfgrass root weight for two 16-week thatch control field studies. Means fo significantly different according to Fisher's protected LSD (a=0.10). The Walker Golf C

Figure 1. Turfgrass root weight for two 16-week thatch control greenhouse studies. Means followed by the same letter within each year are not significantly different according to Fischer's protected LSD at (a=0.10). Clemson University Greenhouse Facility, Clemson, SC. Figure 1. Turfgrass root weight for two 16-week thatch control greenhouse studies. Means followed by the same letter within each year are not significantly different according to Fischer’s protected LSD at (a=0.10). Clemson University Greenhouse Facility, Clemson, SC. i . u f o i h r w e c l u i . ll l i i

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Figure 4. Turfgrass thatch thickness for two 16-week thatch control field studies. Mea according to Fisher's protected LSD (a=0.10). The Walker Golf Course, Clemson, SC. References: Weaver, JR. 2020. Evaluation of Biological Thatch Control on Golf Greens. Dissertation. Clemso (https://tigerprints.clemson.edu/all_dissertations/2610/?utm_source=tigerprints.clem mpaign=PDFCoverPages) Weaver, JR, LB McCarty. 2020. Evaluating biological thatch control in turfgrass. (https://www.g Accessed Jan. 21 st , 2021. References: Weaver, JR. 2020. Evaluation of Biological Thatch Control on Golf Greens. Dissertation. Clemso (https://tigerprints.clemson.edu/all_dissertations/2610/?utm_source=tigerprints.cle mpaign=PDFCoverPages) Weaver, JR, LB McCarty. 2020. Evaluating biological thatch control in turfgrass. (https://www.g Accessed Jan. 21 st , 2021. Figure 4. Turfgrass thatch thickness for two 16-week thatch control field studies. Mea according to Fisher's protected LSD (a=0.10). The Walker Golf Course, Clemson, SC. Figure 4. Turfgrass thatch thickness for two 16-week thatch control field studies. Means followed by the same letter are not significantly different according to Fisher’s prot cted LSD (a=0.10). The Walker Golf Course, Clemson, SC. References: W aver, JR. 2020. Evaluation of Biological Thatch Control on Golf Greens. Dissertation. Clemson University, Clemson S.C. (https://tigerprints.clemson.edu/all_dissertations/2610/?utm_ source=tigerprints.clemson.edu%2Fall_dissertations%2F2610&utm_medium=PDF&utm_ campaign=PDFCoverPages) Weaver, JR, LB McCarty. 2020. Evaluating biological thatch control in turfgrass. (https://www.gcmonline.com/latest-stories/biological-thatch-control-turfgrass). Accessed Jan. 21st, 2021.

Figure 2. Turfgrass rooting length for two 16-week thatch control field studies. Means followed by the same letter within each plot are not significantly different according to Fisher's protected LSD (a=0.10). The Walker Golf Course, Clemson, SC i . s i g l o ch l i l udi . M ll l i i l significa l different ac i i ' . . l l , l , Figure 2. Turfgrass rooting length for two 16-week thatch control field studies. Means followed by the same l tter within each plot are ot significantly different according to Fis er’s protected LSD (a=0.10). The Walker Golf Course, Clemson, SC

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